A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata

Xiaoling Ma; Ningxin Jiang; Jingxian Fu; Yuping Li; Lijun Zhou; Ling Yuan; Ying Wang; Yongqing Li

doi:10.1016/j.jplph.2023.154145

A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata

Xiaoling Ma, Ningxin Jiang, Jingxian Fu, Yuping Li, Lijun Zhou, Ling Yuan, Ying Wang, Yongqing Li

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Licochalcone A (LCA) is a characteristic compound of Glycyrrhiza inflata with anti-inflammatory, antioxidant and antitumor activities. However, G. inflata produces LCA in low quantities that does not meet the market demand. In this study, we found that DNA methylation inhibitor 5-azacitidine (5-azaC) successfully improved the LCA contents in G. inflata seedlings. Transcriptome analysis revealed a series of differentially expressed genes (DEGs), including transcription factors such as MYB, ERF, WRKY, and some structural genes related to flavonoid biosynthesis. However, whole genome bisulfite sequencing (BS-seq) results showed little effect of the 5-azaC treatment on the alteration of DNA methylation on these genes, indicating the possibility that 5-azaC acts as a stimulus, but not an epigenetic modulation factor to improve the LCA content in G. inflata. Additionally, we applied the 5-azaC treatment to field plants and hairy roots and successfully increased the LCA contents in both cases. This research demonstrates the feasibility of 5-azaC treatments in future applications to improve plant production of LCA.

Original language	English
Article number	154145
Journal	Journal of Plant Physiology
Volume	292
DOIs	https://doi.org/10.1016/j.jplph.2023.154145
State	Published - Jan 2024

Bibliographical note

Publisher Copyright:
© 2023 Elsevier GmbH

Funding

This work was supported by Biological Resources Program, Chinese Academy of Sciences (KFJ-BRP-007-017), Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams (20230KJ148), the Foundation of Guangdong Provincial Key Laboratory of Applied Botany (2023B1212060046) & Key Laboratory of South China Agricultural Plant Molecular Analysis and Genetic Improvement (KF200203), South China Botanical Garden, Chinese Academy of Sciences (Granted No: QNXM-02), and China Postdoctoral Science Foundation (2020M682953 and 2021T140678).

Funders	Funder number
Biological Resources Program
Foundation of Guangdong Provincial Key Laboratory of Applied Botany	KF200203, 2023B1212060046
South China Botanical Garden	QNXM-02
Guangdong Provincial Special Fund For Modern Agriculture Industry Technology Innovation Teams	20230KJ148
Chinese Academy of Sciences	KFJ-BRP-007-017
China Postdoctoral Science Foundation	2020M682953, 2021T140678

Keywords

5-Azacitidine
DNA methylation inhibitor
Flavonoid
Glycyrrhiza inflata
Licochalcone A

ASJC Scopus subject areas

Physiology
Agronomy and Crop Science
Plant Science

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10.1016/j.jplph.2023.154145

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title = "A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata",

abstract = "Licochalcone A (LCA) is a characteristic compound of Glycyrrhiza inflata with anti-inflammatory, antioxidant and antitumor activities. However, G. inflata produces LCA in low quantities that does not meet the market demand. In this study, we found that DNA methylation inhibitor 5-azacitidine (5-azaC) successfully improved the LCA contents in G. inflata seedlings. Transcriptome analysis revealed a series of differentially expressed genes (DEGs), including transcription factors such as MYB, ERF, WRKY, and some structural genes related to flavonoid biosynthesis. However, whole genome bisulfite sequencing (BS-seq) results showed little effect of the 5-azaC treatment on the alteration of DNA methylation on these genes, indicating the possibility that 5-azaC acts as a stimulus, but not an epigenetic modulation factor to improve the LCA content in G. inflata. Additionally, we applied the 5-azaC treatment to field plants and hairy roots and successfully increased the LCA contents in both cases. This research demonstrates the feasibility of 5-azaC treatments in future applications to improve plant production of LCA.",

keywords = "5-Azacitidine, DNA methylation inhibitor, Flavonoid, Glycyrrhiza inflata, Licochalcone A",

author = "Xiaoling Ma and Ningxin Jiang and Jingxian Fu and Yuping Li and Lijun Zhou and Ling Yuan and Ying Wang and Yongqing Li",

note = "Publisher Copyright: {\textcopyright} 2023 Elsevier GmbH",

year = "2024",

month = jan,

doi = "10.1016/j.jplph.2023.154145",

language = "English",

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T1 - A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata

AU - Ma, Xiaoling

AU - Jiang, Ningxin

AU - Fu, Jingxian

AU - Li, Yuping

AU - Zhou, Lijun

AU - Yuan, Ling

AU - Wang, Ying

AU - Li, Yongqing

PY - 2024/1

Y1 - 2024/1

N2 - Licochalcone A (LCA) is a characteristic compound of Glycyrrhiza inflata with anti-inflammatory, antioxidant and antitumor activities. However, G. inflata produces LCA in low quantities that does not meet the market demand. In this study, we found that DNA methylation inhibitor 5-azacitidine (5-azaC) successfully improved the LCA contents in G. inflata seedlings. Transcriptome analysis revealed a series of differentially expressed genes (DEGs), including transcription factors such as MYB, ERF, WRKY, and some structural genes related to flavonoid biosynthesis. However, whole genome bisulfite sequencing (BS-seq) results showed little effect of the 5-azaC treatment on the alteration of DNA methylation on these genes, indicating the possibility that 5-azaC acts as a stimulus, but not an epigenetic modulation factor to improve the LCA content in G. inflata. Additionally, we applied the 5-azaC treatment to field plants and hairy roots and successfully increased the LCA contents in both cases. This research demonstrates the feasibility of 5-azaC treatments in future applications to improve plant production of LCA.

AB - Licochalcone A (LCA) is a characteristic compound of Glycyrrhiza inflata with anti-inflammatory, antioxidant and antitumor activities. However, G. inflata produces LCA in low quantities that does not meet the market demand. In this study, we found that DNA methylation inhibitor 5-azacitidine (5-azaC) successfully improved the LCA contents in G. inflata seedlings. Transcriptome analysis revealed a series of differentially expressed genes (DEGs), including transcription factors such as MYB, ERF, WRKY, and some structural genes related to flavonoid biosynthesis. However, whole genome bisulfite sequencing (BS-seq) results showed little effect of the 5-azaC treatment on the alteration of DNA methylation on these genes, indicating the possibility that 5-azaC acts as a stimulus, but not an epigenetic modulation factor to improve the LCA content in G. inflata. Additionally, we applied the 5-azaC treatment to field plants and hairy roots and successfully increased the LCA contents in both cases. This research demonstrates the feasibility of 5-azaC treatments in future applications to improve plant production of LCA.

KW - 5-Azacitidine

KW - DNA methylation inhibitor

KW - Flavonoid

KW - Glycyrrhiza inflata

KW - Licochalcone A

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A cytosine analogue 5-azacitidine improves the accumulation of licochalcone A in licorice Glycyrrhiza inflata

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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