A defect in glyoxysomal fatty acid β-oxidation reduces jasmonic acid accumulation in Arabidopsis

M. M. Afitlhile, H. Fukushige, M. Nishimura, D. F. Hildebrand

Research output: Contribution to journalArticlepeer-review

43 Scopus citations

Abstract

The final steps of jasmonic acid (JA) biosynthesis are thought to involve peroxisomal β-oxidation, but this has not been directly demonstrated. The last and key step in fatty acid β-oxidation is catalyzed by 3-ketoacyl-CoA thiolase (KAT) (EC 2.3.1.16). A mutant of Arabidopsis thaliana ecotype Landsberg erecta, which lacks a functional KAT protein and is defective in glyoxysomal fatty acid β-oxidation has been reported. In this study, the mutant was found to accumulate reduced level of JA in both its wounded cotyledons and leaves, while only the cotyledons accumulate 3-oxo-2-(pent-2′-enyl)- cyclopentane-1-octanoic acid (OPC-8:0). This indicates that a defect in one of the thiolase isoenzymes impairs β-oxidation of OPC-8:0 to JA. The mutant had sufficient thiolase activity for the synthesis of JA in the unwounded but not in the wounded tissues. Activities of the enzymes in the JA pathway that catalyze the steps, which precede β-oxidation were not altered by the mutation in a thiolase protein. Thus, reduced levels of JA in the wounded tissues of the mutant were attributed to the defect in a thiolase protein.

Original languageEnglish
Pages (from-to)603-609
Number of pages7
JournalPlant Physiology and Biochemistry
Volume43
Issue number6
DOIs
StatePublished - Jun 2005

Keywords

  • 12-Oxo-phytodienoic acid
  • Arabidopsis
  • Jasmonic acid
  • OPC-8:0
  • Thiolase
  • ped1
  • β-oxidation

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

Fingerprint

Dive into the research topics of 'A defect in glyoxysomal fatty acid β-oxidation reduces jasmonic acid accumulation in Arabidopsis'. Together they form a unique fingerprint.

Cite this