A device for the simple and rapid transcervical transfer of mouse embryos eliminates the need for surgery and potential post-operative complications

Michael A. Green, Shannon Bass, Brett T. Spear

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

We describe a novel device that can be used for the transcervical transfer of embryos into pseudopregnant female mice. This nonsurgical embryo transfer (NSET) device is as efficient as standard surgical embryo transfer in the production of transgenic mice, and can also be used for the transfer of embryonic stem cell-containing chimeric blastocysts and cryopreserved embryos. In addition to the elimination of surgery, recipient females do not have to be anesthetized. The NSET device eliminates a painful surgical procedure as well as potential complications associated with anesthesia/post-operative care, reduces the technical expertise and equipment needed for surgical transfer, and represents substantial cost savings and regulatory reduction. NSET technology provides an easy and rapid alternative to surgical embryo transfer.

Original languageEnglish
Pages (from-to)919-924
Number of pages6
JournalBioTechniques
Volume47
Issue number5
DOIs
StatePublished - Nov 2009

Keywords

  • Chimeric mice
  • Cryopreservation
  • Embryo transfer
  • Embryonic stem cells
  • Mouse surgery
  • Transgenic mice

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry, Genetics and Molecular Biology (all)

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