TY - JOUR
T1 - A FLUOROMETRIC ASSAY FOR CHOLINE ACETYLTRANSFERASE and ITS USE IN THE PURIFICATION OF THE ENZYME FROM HUMAN PLACENTA
AU - Hersh, Louis B.
AU - Coe, Barbara
AU - Casey, Lynette
PY - 1978/5
Y1 - 1978/5
N2 - Abstract— A fluorometric assay for choline acetyltransferase has been developed. This assay is based on coupling the choline acetyltransferase dependent formation of acetyl‐CoA from acetylcholine and coenzyme A, to the reactions catalyzed by the enzymes citrate synthase and malic dehydrogenase. Although this assay is not as sensitive as previously described radiometric assays, it can be conveniently used during enzyme purification. Employing this assay method, choline acetyltransferase has been purified from human placenta to a specific activity of 92.7 μmol acetylcholine formed/min/mg protein.
AB - Abstract— A fluorometric assay for choline acetyltransferase has been developed. This assay is based on coupling the choline acetyltransferase dependent formation of acetyl‐CoA from acetylcholine and coenzyme A, to the reactions catalyzed by the enzymes citrate synthase and malic dehydrogenase. Although this assay is not as sensitive as previously described radiometric assays, it can be conveniently used during enzyme purification. Employing this assay method, choline acetyltransferase has been purified from human placenta to a specific activity of 92.7 μmol acetylcholine formed/min/mg protein.
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U2 - 10.1111/j.1471-4159.1978.tb12401.x
DO - 10.1111/j.1471-4159.1978.tb12401.x
M3 - Article
C2 - 660185
AN - SCOPUS:0018098888
SN - 0022-3042
VL - 30
SP - 1077
EP - 1085
JO - Journal of Neurochemistry
JF - Journal of Neurochemistry
IS - 5
ER -