A FRET-based approach for identification of proteasome catalytic subunit composition

Ji Eun Park, Ying Wu, Kimberly Cornish Carmony, Zachary Miller, Lalit Kumar Sharma, Do Min Lee, Doo Young Kim, Wooin Lee, Kyung Bo Kim

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

Mammalian cells have two main types of proteasomes, the constitutive proteasome and the immunoproteasome, each containing a distinct set of three catalytic subunits. Recently, additional proteasome subtypes containing a non-standard mixture of catalytic subunits have gained increasing attention, especially due to their presence in cancer settings. However, practical methods for identifying proteasome subtypes have been lacking. Here, we report the development of the first fluorescence resonance energy transfer.

Original languageEnglish
Pages (from-to)196-200
Number of pages5
JournalMolecular BioSystems
Volume10
Issue number2
DOIs
StatePublished - Feb 2014

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology

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