TY - JOUR
T1 - A FRET-based approach for identification of proteasome catalytic subunit composition
AU - Park, Ji Eun
AU - Wu, Ying
AU - Carmony, Kimberly Cornish
AU - Miller, Zachary
AU - Sharma, Lalit Kumar
AU - Lee, Do Min
AU - Kim, Doo Young
AU - Lee, Wooin
AU - Kim, Kyung Bo
PY - 2014/2
Y1 - 2014/2
N2 - Mammalian cells have two main types of proteasomes, the constitutive proteasome and the immunoproteasome, each containing a distinct set of three catalytic subunits. Recently, additional proteasome subtypes containing a non-standard mixture of catalytic subunits have gained increasing attention, especially due to their presence in cancer settings. However, practical methods for identifying proteasome subtypes have been lacking. Here, we report the development of the first fluorescence resonance energy transfer.
AB - Mammalian cells have two main types of proteasomes, the constitutive proteasome and the immunoproteasome, each containing a distinct set of three catalytic subunits. Recently, additional proteasome subtypes containing a non-standard mixture of catalytic subunits have gained increasing attention, especially due to their presence in cancer settings. However, practical methods for identifying proteasome subtypes have been lacking. Here, we report the development of the first fluorescence resonance energy transfer.
UR - http://www.scopus.com/inward/record.url?scp=84891384603&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84891384603&partnerID=8YFLogxK
U2 - 10.1039/c3mb70471h
DO - 10.1039/c3mb70471h
M3 - Article
C2 - 24301521
AN - SCOPUS:84891384603
SN - 1742-206X
VL - 10
SP - 196
EP - 200
JO - Molecular BioSystems
JF - Molecular BioSystems
IS - 2
ER -
