A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

Shanteri Singh; Pauline Peltier-Pain; Marco Tonelli; Jon S. Thorson

doi:10.1021/ol501241a

A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

Shanteri Singh, Pauline Peltier-Pain, Marco Tonelli, Jon S. Thorson

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively ¹³C-labeled sugar nucleotides and inverse ¹³C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.

Original language	English
Pages (from-to)	3220-3223
Number of pages	4
Journal	Organic Letters
Volume	16
Issue number	12
DOIs	https://doi.org/10.1021/ol501241a
State	Published - Jun 20 2014

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

Access to Document

10.1021/ol501241a

Cite this

@article{9dec4e2d0020437db9afacfc554604bc,

title = "A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways",

abstract = "A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively 13C-labeled sugar nucleotides and inverse 13C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.",

author = "Shanteri Singh and Pauline Peltier-Pain and Marco Tonelli and Thorson, {Jon S.}",

year = "2014",

month = jun,

day = "20",

doi = "10.1021/ol501241a",

language = "English",

volume = "16",

pages = "3220--3223",

number = "12",

}

TY - JOUR

T1 - A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

AU - Singh, Shanteri

AU - Peltier-Pain, Pauline

AU - Tonelli, Marco

AU - Thorson, Jon S.

PY - 2014/6/20

Y1 - 2014/6/20

N2 - A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively 13C-labeled sugar nucleotides and inverse 13C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.

AB - A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively 13C-labeled sugar nucleotides and inverse 13C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.

UR - http://www.scopus.com/inward/record.url?scp=84903211080&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84903211080&partnerID=8YFLogxK

U2 - 10.1021/ol501241a

DO - 10.1021/ol501241a

M3 - Article

C2 - 24911465

AN - SCOPUS:84903211080

SN - 1523-7060

VL - 16

SP - 3220

EP - 3223

JO - Organic Letters

JF - Organic Letters

IS - 12

ER -

A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this