A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

Shanteri Singh; Pauline Peltier-Pain; Marco Tonelli; Jon S. Thorson

doi:10.1021/ol501241a

A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

Shanteri Singh
, Pauline Peltier-Pain
, Marco Tonelli
, Jon S. Thorson

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively ¹³C-labeled sugar nucleotides and inverse ¹³C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.

Original language	English
Pages (from-to)	3220-3223
Number of pages	4
Journal	Organic Letters
Volume	16
Issue number	12
DOIs	https://doi.org/10.1021/ol501241a
State	Published - Jun 20 2014

Funding

Funders	Funder number
National Center for Advancing Translational Sciences (NCATS)	UL1TR000117
National Institutes of Health (NIH)	RR02781, RR08438, P41GM10399, P41RR02301, RO1 CA84374, R37 AI52218
National Stroke Foundation	DMB-8415048, BIR-9214394, OIA-9977486
U.S. Department of Agriculture
Michigan State University-U.S. Department of Energy (MSU-DOE) Plant Research Laboratory
National Institute of General Medical Sciences	F32GM010399

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry
Organic Chemistry

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10.1021/ol501241a

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title = "A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways",

abstract = "A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively 13C-labeled sugar nucleotides and inverse 13C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.",

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T1 - A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

AU - Singh, Shanteri

AU - Peltier-Pain, Pauline

AU - Tonelli, Marco

AU - Thorson, Jon S.

PY - 2014/6/20

Y1 - 2014/6/20

N2 - A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively 13C-labeled sugar nucleotides and inverse 13C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.

AB - A simple method for the study of sugar-nucleotide-dependent multienzyme cascades is highlighted where the use of selectively 13C-labeled sugar nucleotides and inverse 13C detection NMR offers fast, direct detection and quantification of reactants and products and circumvents the need for chromatographic separation. The utility of the method has been demonstrated by characterizing four previously uncharacterized sugar nucleotide biosynthetic enzymes involved in calicheamicin biosynthesis.

UR - https://www.scopus.com/pages/publications/84903211080

UR - https://www.scopus.com/pages/publications/84903211080#tab=citedBy

U2 - 10.1021/ol501241a

DO - 10.1021/ol501241a

M3 - Article

C2 - 24911465

AN - SCOPUS:84903211080

SN - 1523-7060

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JO - Organic Letters

JF - Organic Letters

IS - 12

ER -

A general NMR-based strategy for the in Situ characterization of sugar-nucleotide-dependent biosynthetic pathways

Abstract

Funding

ASJC Scopus subject areas

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