A guide to electron paramagnetic resonance spectroscopy of Photosystem II membranes

Anne Frances Miller, Gary W. Brudvig

Research output: Contribution to journalReview articlepeer-review

78 Scopus citations


This guide is intended to aid in the detection and identification of paramagnetic species in Photosystem II membranes, by electron paramagnetic resonance spectroscopy. The spectral features and occurrence of each of the electron paramagnetic resonance signals from Photosystem II are discussed, in relation to the nature of the moiety giving rise to the signal and the role of that species in photosynthetic electron transport. Examples of most of the signals discussed are shown. The electron paramagnetic resonance signals produced by the cytochrome b6f and Photosystem I complexes, as well as the signals from other common contaminants, are also reviewed. Furthermore, references to seminal experiments on bacterial reaction centers are included. By reviewing both the spectroscopic and biochemical bases for the electron paramagnetic resonance signals of the cofactors that mediate photosynthetic electron transport, this paper provides an introduction to the use and interpretation of electron paramagnetic resonance spectroscopy in the study of Photosystem II.

Original languageEnglish
Pages (from-to)1-18
Number of pages18
JournalBiochimica et Biophysica Acta - Bioenergetics
Issue number1
StatePublished - Jan 3 1991

Bibliographical note

Funding Information:
This work was supported by the National Institutes of Health (GM327i5). GWB is the recipient of a Camille and Henry Dreyfus Teacher/Scholarship. The authors would like to thank J.B. Innes for an EPR spectrum and A.W. Rutherford for helpful discussions, a preprint of a paper in press and permission to use two figures from his published work in this review.


  • EPR
  • Photosystem II

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology


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