A high-throughput approach for studying virus replication in yeast

Judit Pogany, Tadas Panavas, Elena Serviene, Muhammad Shah Nawaz-ul-Rehman, Peter D. Nagy

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Viruses are intracellular pathogens that are dependent on viral and host factors for multiplication. Model hosts, such as yeast, can be very valuable in identifying host factors involved in viral replication. Yeast is also useful for studies on functional interactions of host factors with viral proteins and/or virus nucleic acids. The advantages of using yeast include the availability of a single gene-deletion library and the essential gene library (yTHC); the controllable small- or large-scale expression of viral proteins and nucleic acids; and the rapid growth of yeast strains. Procedures that facilitate high-throughput analysis of host factors and plant and animal RNA virus replication in yeast, with a plant virus (tombusvirus; TBSV) and an animal virus (nodavirus; FHV) as examples, are described.

Original languageEnglish
Article number16J.1
JournalCurrent Protocols in Microbiology
Issue numberSUPPL.19
DOIs
StatePublished - Nov 2010

Keywords

  • High-throughput
  • Host factors
  • Nodaviruses
  • Northern blotting
  • Tombusviruses
  • Transformation
  • Viral RNA extraction
  • Yeast

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

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