TY - JOUR
T1 - A host-factor interaction and localization map for a plant-adapted rhabdovirus implicates cytoplasm- tethered transcription activators in cell-to-cell movement
AU - Min, Byoung Eun
AU - Martin, Kathleen
AU - Wang, Renyuan
AU - Tafelmeyer, Petra
AU - Bridges, Max
AU - Goodin, Michael
PY - 2010/11
Y1 - 2010/11
N2 - To identify host factors that play critical roles in processes, including cell-to-cell movement of plant-adapted rhabdovi- ruses, we constructed and validated a high-resolution Nico- tiana benthamiana yeast two-hybrid library. The library was screened with the putative movement protein (sc 4), nu- cleocapsid (N), and matrix (M) proteins of Sonchus yellow net virus (SYNV). This resulted in identification of 31 potential host factors. Steady-state localization studies using autofluorescent protein fusions to full-length clones of inter- actors were conducted in transgenic N. benthamiana marker lines. Bimolecular fluorescence complementation assays were used to validate two-hybrid interactions. The sc4 inter- actor, sc4i21, localized to microtubules. The N interactor, Ni67, localized to punctuate loci on the endoplasmic reticulum. These two proteins are 84% identical homologues of the Arabidopsis phloem-associated transcription activator AtVOZl, and contain functional nuclear localization signals. Sc4i17 is a microtubule-associated motor protein. The M interactor, Mi7, is a nuclear-localized transcription factor. Combined with a binary interaction map for SYNV proteins, our data support a model in which the SYNV nu- cleocapsids are exported from the nucleus and moved cell- to-cell by transcription activators tethered in the cytoplasm.
AB - To identify host factors that play critical roles in processes, including cell-to-cell movement of plant-adapted rhabdovi- ruses, we constructed and validated a high-resolution Nico- tiana benthamiana yeast two-hybrid library. The library was screened with the putative movement protein (sc 4), nu- cleocapsid (N), and matrix (M) proteins of Sonchus yellow net virus (SYNV). This resulted in identification of 31 potential host factors. Steady-state localization studies using autofluorescent protein fusions to full-length clones of inter- actors were conducted in transgenic N. benthamiana marker lines. Bimolecular fluorescence complementation assays were used to validate two-hybrid interactions. The sc4 inter- actor, sc4i21, localized to microtubules. The N interactor, Ni67, localized to punctuate loci on the endoplasmic reticulum. These two proteins are 84% identical homologues of the Arabidopsis phloem-associated transcription activator AtVOZl, and contain functional nuclear localization signals. Sc4i17 is a microtubule-associated motor protein. The M interactor, Mi7, is a nuclear-localized transcription factor. Combined with a binary interaction map for SYNV proteins, our data support a model in which the SYNV nu- cleocapsids are exported from the nucleus and moved cell- to-cell by transcription activators tethered in the cytoplasm.
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U2 - 10.1094/MPMI-04-10-0097
DO - 10.1094/MPMI-04-10-0097
M3 - Article
C2 - 20923350
AN - SCOPUS:78149314668
SN - 0894-0282
VL - 23
SP - 1420
EP - 1432
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 11
ER -