A light-activated metal complex targets both DNA and RNA in a fluorescent in vitro transcription and translation assay

David K. Heidary, Edith C. Glazer

Research output: Contribution to journalArticlepeer-review

16 Scopus citations

Abstract

A coupled in vitro transcription and translation (IVTT) assay that uses GFP as a fluorescent reporter allowed the potency of a light-activated cytotoxic ruthenium agent to be quantified. The compound inhibits the function of both DNA and mRNA only upon light activation. The IVTT functional assay provides estimates of potency that are consistent with cellular cytotoxicity values, in marked contrast to the values obtained from traditional DNA-damage assays. Lost in translation? A coupled in vitro transcription and translation (IVTT) assay allowed the quantification of the potency of a light-activated cytotoxic ruthenium agent (red). This compound inhibits the functions of both DNA and mRNA, only upon light activation. The IVTT assay provides a better estimate of potency (consistent with cellular cytotoxicity values) than traditional DNA-damage assays.

Original languageEnglish
Pages (from-to)507-511
Number of pages5
JournalChembiochem : a European journal of chemical biology
Volume15
Issue number4
DOIs
StatePublished - Mar 3 2014

Keywords

  • cancer
  • cisplatin
  • drug discovery
  • high-throughput screening
  • ruthenium

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Fingerprint

Dive into the research topics of 'A light-activated metal complex targets both DNA and RNA in a fluorescent in vitro transcription and translation assay'. Together they form a unique fingerprint.

Cite this