TY - GEN
T1 - A low temperature cofired ceramic microfluidic calorimeter for ELISA biosensing
AU - Brummel, Benjamin
AU - Berron, Bradley
AU - Eitel, Richard E.
PY - 2013
Y1 - 2013
N2 - The expense and difficulty of current biomarker detection methods is driving the design of microfluidic detection platforms. A ceramic microfluidic biosensor has been developed for conducting enzyme-linked immunosorbent assays (ELISA) using a novel polymerization amplified thermal detection (PATD) scheme. Prototype testing has yielded several results that support the viability of this device. It was seen that LTCC is an effective and durable substrate for the temperature sensor. Noise testing with our prototype revealed that our temperature sensors can detect changes as small as 0.01 K. Additional temperature testing showed that the thermistor behavior matches the expected thermistor beta equation. Finally, it was shown that polymerization reaction induction time is inversely proportional to glucose oxidase (GOx) initiator concentration. These preliminary results provide a foundation for future work developing the sensor into a protein detection device with cancer prognosis applications.
AB - The expense and difficulty of current biomarker detection methods is driving the design of microfluidic detection platforms. A ceramic microfluidic biosensor has been developed for conducting enzyme-linked immunosorbent assays (ELISA) using a novel polymerization amplified thermal detection (PATD) scheme. Prototype testing has yielded several results that support the viability of this device. It was seen that LTCC is an effective and durable substrate for the temperature sensor. Noise testing with our prototype revealed that our temperature sensors can detect changes as small as 0.01 K. Additional temperature testing showed that the thermistor behavior matches the expected thermistor beta equation. Finally, it was shown that polymerization reaction induction time is inversely proportional to glucose oxidase (GOx) initiator concentration. These preliminary results provide a foundation for future work developing the sensor into a protein detection device with cancer prognosis applications.
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UR - http://www.scopus.com/inward/citedby.url?scp=84901942757&partnerID=8YFLogxK
M3 - Conference contribution
AN - SCOPUS:84901942757
SN - 9781629937182
T3 - 9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013
SP - 168
EP - 172
BT - 9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013
T2 - 9th IMAPS/ACerS International Conference and Exhibition on Ceramic Interconnect and Ceramic Microsystems Technologies, CICMT 2013
Y2 - 23 April 2013 through 25 April 2013
ER -
