A micromethod for the measurement of acetyl phosphate and acetyl coenzyme A

Arthur G. Hunt; Jen shiang Hong

doi:10.1016/0003-2697(80)90586-2

A micromethod for the measurement of acetyl phosphate and acetyl coenzyme A

Arthur G. Hunt, Jen shiang Hong

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

A method for the determination of acetyl phosphate in cell-free extracts is described. The sensitivity of this method is 20 pmol/ml of extract. An adaptation that permits the measurement of picomole quantities of acetyl CoA is also presented.

Original language	English
Pages (from-to)	290-294
Number of pages	5
Journal	Analytical Biochemistry
Volume	108
Issue number	2
DOIs	https://doi.org/10.1016/0003-2697(80)90586-2
State	Published - 1980

Bibliographical note

Funding Information:
This work was supported by Grant GM22576 from the National Institute of General Medical Sciences. A. G. H. is a predoctoral trainee supported by Training Grant GM212 from the National Institute of General Medical Sciences. J. -s. H. is a NIH Research Career Development Awardee (K04-GM-0092). This is publication No. 1336 from the Department of Biochemistry, Brandeis University.

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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title = "A micromethod for the measurement of acetyl phosphate and acetyl coenzyme A",

abstract = "A method for the determination of acetyl phosphate in cell-free extracts is described. The sensitivity of this method is 20 pmol/ml of extract. An adaptation that permits the measurement of picomole quantities of acetyl CoA is also presented.",

author = "Hunt, {Arthur G.} and Hong, {Jen shiang}",

note = "Funding Information: This work was supported by Grant GM22576 from the National Institute of General Medical Sciences. A. G. H. is a predoctoral trainee supported by Training Grant GM212 from the National Institute of General Medical Sciences. J. -s. H. is a NIH Research Career Development Awardee (K04-GM-0092). This is publication No. 1336 from the Department of Biochemistry, Brandeis University.",

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AU - Hunt, Arthur G.

AU - Hong, Jen shiang

N1 - Funding Information: This work was supported by Grant GM22576 from the National Institute of General Medical Sciences. A. G. H. is a predoctoral trainee supported by Training Grant GM212 from the National Institute of General Medical Sciences. J. -s. H. is a NIH Research Career Development Awardee (K04-GM-0092). This is publication No. 1336 from the Department of Biochemistry, Brandeis University.

PY - 1980

Y1 - 1980

N2 - A method for the determination of acetyl phosphate in cell-free extracts is described. The sensitivity of this method is 20 pmol/ml of extract. An adaptation that permits the measurement of picomole quantities of acetyl CoA is also presented.

AB - A method for the determination of acetyl phosphate in cell-free extracts is described. The sensitivity of this method is 20 pmol/ml of extract. An adaptation that permits the measurement of picomole quantities of acetyl CoA is also presented.

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A micromethod for the measurement of acetyl phosphate and acetyl coenzyme A

Abstract

Bibliographical note

ASJC Scopus subject areas

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