A multiple antibiotic-resistant Enterobacter cloacae strain isolated from a bioethanol fermentation facility

Colin A. Murphree; Qing Li; E. Patrick Heist; Luke A. Moe

doi:10.1264/jsme2.ME13162

A multiple antibiotic-resistant Enterobacter cloacae strain isolated from a bioethanol fermentation facility

Colin A. Murphree, Qing Li, E. Patrick Heist, Luke A. Moe

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

An Enterobacter cloacae strain (E. cloacae F3S3) that was collected as part of a project to assess antibiotic resistance among bacteria isolated from bioethanol fermentation facilities demonstrated high levels of resistance to antibiotics added prophylactically to bioethanol fermentors. PCR assays revealed the presence of canonical genes encoding resistance to penicillin (ampC) and erythromycin (ermG). Assays measuring biofilm formation under antibiotic stress indicated that erythromycin induced biofilm formation in E. cloacae F3S3. Planktonic growth and biofilm formation were observed at a high ethanol content, indicating E. cloacae F3S3 can persist in a bioethanol fermentor under the highly variable environmental conditions found in fermentors.

Original language	English
Pages (from-to)	322-325
Number of pages	4
Journal	Microbes and Environments
Volume	29
Issue number	3
DOIs	https://doi.org/10.1264/jsme2.ME13162
State	Published - Sep 17 2014

Bibliographical note

Publisher Copyright:
© 2014 Microbes Environ. All rights reserved.

Keywords

Antibiotic resistance
Bioethanol
Enterobacter cloacae
Fermentation

ASJC Scopus subject areas

Ecology, Evolution, Behavior and Systematics
Soil Science
Plant Science

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10.1264/jsme2.ME13162

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abstract = "An Enterobacter cloacae strain (E. cloacae F3S3) that was collected as part of a project to assess antibiotic resistance among bacteria isolated from bioethanol fermentation facilities demonstrated high levels of resistance to antibiotics added prophylactically to bioethanol fermentors. PCR assays revealed the presence of canonical genes encoding resistance to penicillin (ampC) and erythromycin (ermG). Assays measuring biofilm formation under antibiotic stress indicated that erythromycin induced biofilm formation in E. cloacae F3S3. Planktonic growth and biofilm formation were observed at a high ethanol content, indicating E. cloacae F3S3 can persist in a bioethanol fermentor under the highly variable environmental conditions found in fermentors.",

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A multiple antibiotic-resistant Enterobacter cloacae strain isolated from a bioethanol fermentation facility

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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