Abstract
Ligation of the left anterior descending (LAD) coronary artery in the mouse heart is a widely used model to simulate myocardial infarction and ischemia–reperfusion injury. Here we describe a ligation technique routinely performed in our laboratory to induce myocardial infarction that may be used to study ischemia–reperfusion injury in the myocardium. The methods described enhance location of the LAD coronary artery to allow for accurate ligation, thus increasing reproducibility of infarct size and location.
Original language | English |
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Title of host publication | Methods in Molecular Biology |
Pages | 145-153 |
Number of pages | 9 |
DOIs | |
State | Published - 2018 |
Publication series
Name | Methods in Molecular Biology |
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Volume | 1717 |
ISSN (Print) | 1064-3745 |
Bibliographical note
Publisher Copyright:© 2018, Springer Science+Business Media, LLC.
Keywords
- LAD ligation
- Left anterior descending coronary artery
- Myocardial infarction
- Myocardial ischemia–reperfusion
- Permanent ligation
ASJC Scopus subject areas
- Molecular Biology
- Genetics