Abstract
The highly specific recognition of a natural cytokinin, trans-zeatin, by cytokinin-binding protein (CBP) of 67 kDa from barley leaves was detected with an assay developed on the basis of cytokinin competition in ELISA with anti-idiotype antibodies (raised against antibodies to zeatin) for complex formation with CBP. Monoclonal antibodies (mAbs) raised against 70 kDa CBP from etiolated maize seedlings cross-reacted with barley 67 kDa CBP and prevented barley CBP and trans-zeatin induced activation of transcription elongation directed by RNA polymerase I associated with barley chromatin. One mAb (Z-6) had an agonistic effect. Maize CBP replaced barley CBP in activation of RNA synthesis with cytokinin in the barley transcription system. Hence, a new family of cytokinin receptors with common functions and immunodeterminants including maize and barley CBPs was found.
| Original language | English |
|---|---|
| Pages (from-to) | 239-242 |
| Number of pages | 4 |
| Journal | FEBS Letters |
| Volume | 423 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 20 1998 |
Bibliographical note
Funding Information:We are very grateful to Dr. J. Corse for the gift of zeatin- O -glucoside. This work was partially supported by the Russian Foundation for Fundamental Research (Grants 96-04-50705, 96-04-00128 G and 96-15-98030) and by INTAS Grant 93-678 EXT.
Keywords
- Anti-idiotype antibody
- Chromatin
- Cytokinin-binding protein
- Monoclonal antibody
- RNA polymerase I
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology