A new family of cytokinin receptors from cereales

O. N. Kulaeva, T. K. Zagranichnaya, F. A. Brovko, N. N. Karavaiko, S. Yu Selivankina, Ya V. Zemlyachenko, M. Hall, V. M. Lipkin, Kh M. Boziev

Research output: Contribution to journalArticlepeer-review

25 Scopus citations

Abstract

The highly specific recognition of a natural cytokinin, trans-zeatin, by cytokinin-binding protein (CBP) of 67 kDa from barley leaves was detected with an assay developed on the basis of cytokinin competition in ELISA with anti-idiotype antibodies (raised against antibodies to zeatin) for complex formation with CBP. Monoclonal antibodies (mAbs) raised against 70 kDa CBP from etiolated maize seedlings cross-reacted with barley 67 kDa CBP and prevented barley CBP and trans-zeatin induced activation of transcription elongation directed by RNA polymerase I associated with barley chromatin. One mAb (Z-6) had an agonistic effect. Maize CBP replaced barley CBP in activation of RNA synthesis with cytokinin in the barley transcription system. Hence, a new family of cytokinin receptors with common functions and immunodeterminants including maize and barley CBPs was found.

Original languageEnglish
Pages (from-to)239-242
Number of pages4
JournalFEBS Letters
Volume423
Issue number2
DOIs
StatePublished - Feb 20 1998

Bibliographical note

Funding Information:
We are very grateful to Dr. J. Corse for the gift of zeatin- O -glucoside. This work was partially supported by the Russian Foundation for Fundamental Research (Grants 96-04-50705, 96-04-00128 G and 96-15-98030) and by INTAS Grant 93-678 EXT.

Keywords

  • Anti-idiotype antibody
  • Chromatin
  • Cytokinin-binding protein
  • Monoclonal antibody
  • RNA polymerase I

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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