A new method for quantitative determination of poly-3-hydroxybutyrate and 3-hydroxybutyrate-3-hydroxyvalerate copolymer in microbial biomass by reversed-phase high-performance liquid chromatography

N. A. Korotkova; V. V. Ashin; N. V. Doronina; Yu A. Trotsenko

A new method for quantitative determination of poly-3-hydroxybutyrate and 3-hydroxybutyrate-3-hydroxyvalerate copolymer in microbial biomass by reversed-phase high-performance liquid chromatography

N. A. Korotkova, V. V. Ashin, N. V. Doronina, Yu A. Trotsenko

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

A new sensitive method for quantitative determination of poly-3-hydroxybutyrate (PHB) and 3-hydroxybutyrate-3-hydroxyvalerate copolymer (PHBV) in microbial biomass by isocratic reversed-phase high-performance liquid chromatography was developed. The method employs chromatographic analysis of crotonic acid formed by PHB hydrolysis with concentrated sulfuric acid or crotonic and methylcrotonic acids formed by PHBV hydrolysis with 1 N NaOH.

Original language	English
Pages (from-to)	302-305
Number of pages	4
Journal	Applied Biochemistry and Microbiology
Volume	33
Issue number	3
State	Published - May 1997

ASJC Scopus subject areas

Biochemistry
Applied Microbiology and Biotechnology

Cite this

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title = "A new method for quantitative determination of poly-3-hydroxybutyrate and 3-hydroxybutyrate-3-hydroxyvalerate copolymer in microbial biomass by reversed-phase high-performance liquid chromatography",

abstract = "A new sensitive method for quantitative determination of poly-3-hydroxybutyrate (PHB) and 3-hydroxybutyrate-3-hydroxyvalerate copolymer (PHBV) in microbial biomass by isocratic reversed-phase high-performance liquid chromatography was developed. The method employs chromatographic analysis of crotonic acid formed by PHB hydrolysis with concentrated sulfuric acid or crotonic and methylcrotonic acids formed by PHBV hydrolysis with 1 N NaOH.",

author = "Korotkova, {N. A.} and Ashin, {V. V.} and Doronina, {N. V.} and Trotsenko, {Yu A.}",

year = "1997",

month = may,

language = "English",

volume = "33",

pages = "302--305",

journal = "Applied Biochemistry and Microbiology",

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A new method for quantitative determination of poly-3-hydroxybutyrate and 3-hydroxybutyrate-3-hydroxyvalerate copolymer in microbial biomass by reversed-phase high-performance liquid chromatography. / Korotkova, N. A.; Ashin, V. V.; Doronina, N. V. et al.
In: Applied Biochemistry and Microbiology, Vol. 33, No. 3, 05.1997, p. 302-305.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A new method for quantitative determination of poly-3-hydroxybutyrate and 3-hydroxybutyrate-3-hydroxyvalerate copolymer in microbial biomass by reversed-phase high-performance liquid chromatography

AU - Korotkova, N. A.

AU - Ashin, V. V.

AU - Doronina, N. V.

AU - Trotsenko, Yu A.

PY - 1997/5

Y1 - 1997/5

N2 - A new sensitive method for quantitative determination of poly-3-hydroxybutyrate (PHB) and 3-hydroxybutyrate-3-hydroxyvalerate copolymer (PHBV) in microbial biomass by isocratic reversed-phase high-performance liquid chromatography was developed. The method employs chromatographic analysis of crotonic acid formed by PHB hydrolysis with concentrated sulfuric acid or crotonic and methylcrotonic acids formed by PHBV hydrolysis with 1 N NaOH.

AB - A new sensitive method for quantitative determination of poly-3-hydroxybutyrate (PHB) and 3-hydroxybutyrate-3-hydroxyvalerate copolymer (PHBV) in microbial biomass by isocratic reversed-phase high-performance liquid chromatography was developed. The method employs chromatographic analysis of crotonic acid formed by PHB hydrolysis with concentrated sulfuric acid or crotonic and methylcrotonic acids formed by PHBV hydrolysis with 1 N NaOH.

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M3 - Article

AN - SCOPUS:0031526221

SN - 0003-6838

VL - 33

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JO - Applied Biochemistry and Microbiology

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ER -

A new method for quantitative determination of poly-3-hydroxybutyrate and 3-hydroxybutyrate-3-hydroxyvalerate copolymer in microbial biomass by reversed-phase high-performance liquid chromatography

Abstract

ASJC Scopus subject areas

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