Abstract
Background: Although elevated serum levels of visinin-like protein 1 (VILIP-1), a neuron-specific calcium sensor protein, are associated with ischaemic stroke, only a single study has evaluated VILIP-1 as a biomarker of traumatic brain injury (TBI). The current proof-of-concept study was designed to determine whether serum VILIP-1 levels increase post-injury in a well-characterized rat unilateral cortical contusion model. Methods: Lateral flow devices (LFDs) rapidly (< 20 min) detected trace serum levels (pg/mL) of VILIP-1 in a small input sample volume (10 µL). Temporal profiles of serum levels at baseline and post-injury were measured in male Sprague Dawley rats subjected to very mild-, mild unilateral-cortical contusion, or naïve surgery and in male Sprague Dawley rats following a diffuse TBI or sham surgery. Results: Mean serum levels were significantly elevated by 0.5 h post-injury and remained so throughout the temporal profile compared with baseline in very mild and mild unilateral contusions but not in naïve surgeries. Serum levels were also elevated in a small cohort of animals subjected to a diffuse TBI injury. Conclusions: Overall, the current study demonstrates that the novel LFD is a reliable and rapid point-of-care diagnostic for the detection and quantification of serum levels of UB-VILIP-1 in a clinically relevant time frame.
Original language | English |
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Pages (from-to) | 363-380 |
Number of pages | 18 |
Journal | Brain Injury |
Volume | 32 |
Issue number | 3 |
DOIs | |
State | Published - Feb 23 2018 |
Bibliographical note
Publisher Copyright:© 2017 Taylor & Francis Group, LLC.
Keywords
- Biomarker
- head injury
- mild brain injury
- traumatic brain injury
ASJC Scopus subject areas
- Neuroscience (miscellaneous)
- Developmental and Educational Psychology
- Clinical Neurology