A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells

Peter T. Nelson; Mariangels De Planell-Saguer; Stella Lamprinaki; Marianthi Kiriakidou; Paul Zhang; Una O'Doherty; Zissimos Mourelatos

doi:10.1261/rna.646007

A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells

Peter T. Nelson, Mariangels De Planell-Saguer, Stella Lamprinaki, Marianthi Kiriakidou, Paul Zhang, Una O'Doherty, Zissimos Mourelatos

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

Argonaute (Ago) proteins bind to microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Currently, there is a paucity of reliable antibodies against mammalian Ago proteins, thus precluding studies of endogenous Ago proteins from tissues. Here we report the development of 2A8, a novel anti-Ago monoclonal antibody that recognizes human and mouse Ago proteins and efficiently immunoprecipitates miRNAs. We report the characterization of 2A8 and its use to clone miRNAs from human brain and from preparations of human polymorphonuclear leukocytes (neutrophils), which revealed a prevalent miRNA with unusual features. Published by Cold Spring Harbor Laboratory Press.

Original language	English
Pages (from-to)	1787-1792
Number of pages	6
Journal	RNA
Volume	13
Issue number	10
DOIs	https://doi.org/10.1261/rna.646007
State	Published - Oct 2007

Funding

Funders	Funder number
National Institute of Neurological Disorders and Stroke	R21NS053839

Keywords

Ago
Antibody
Argonaute
RNAi
miRNA
microRNA

ASJC Scopus subject areas

Molecular Biology

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10.1261/rna.646007

Cite this

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title = "A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells",

abstract = "Argonaute (Ago) proteins bind to microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Currently, there is a paucity of reliable antibodies against mammalian Ago proteins, thus precluding studies of endogenous Ago proteins from tissues. Here we report the development of 2A8, a novel anti-Ago monoclonal antibody that recognizes human and mouse Ago proteins and efficiently immunoprecipitates miRNAs. We report the characterization of 2A8 and its use to clone miRNAs from human brain and from preparations of human polymorphonuclear leukocytes (neutrophils), which revealed a prevalent miRNA with unusual features. Published by Cold Spring Harbor Laboratory Press.",

keywords = "Ago, Antibody, Argonaute, RNAi, miRNA, microRNA",

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doi = "10.1261/rna.646007",

language = "English",

volume = "13",

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T1 - A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells

AU - Nelson, Peter T.

AU - De Planell-Saguer, Mariangels

AU - Lamprinaki, Stella

AU - Kiriakidou, Marianthi

AU - Zhang, Paul

AU - O'Doherty, Una

AU - Mourelatos, Zissimos

PY - 2007/10

Y1 - 2007/10

N2 - Argonaute (Ago) proteins bind to microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Currently, there is a paucity of reliable antibodies against mammalian Ago proteins, thus precluding studies of endogenous Ago proteins from tissues. Here we report the development of 2A8, a novel anti-Ago monoclonal antibody that recognizes human and mouse Ago proteins and efficiently immunoprecipitates miRNAs. We report the characterization of 2A8 and its use to clone miRNAs from human brain and from preparations of human polymorphonuclear leukocytes (neutrophils), which revealed a prevalent miRNA with unusual features. Published by Cold Spring Harbor Laboratory Press.

AB - Argonaute (Ago) proteins bind to microRNA (miRNAs) and short interfering RNAs (siRNAs) and form the core components of effector complexes that mediate miRNA and siRNA function. Currently, there is a paucity of reliable antibodies against mammalian Ago proteins, thus precluding studies of endogenous Ago proteins from tissues. Here we report the development of 2A8, a novel anti-Ago monoclonal antibody that recognizes human and mouse Ago proteins and efficiently immunoprecipitates miRNAs. We report the characterization of 2A8 and its use to clone miRNAs from human brain and from preparations of human polymorphonuclear leukocytes (neutrophils), which revealed a prevalent miRNA with unusual features. Published by Cold Spring Harbor Laboratory Press.

KW - Ago

KW - Antibody

KW - Argonaute

KW - RNAi

KW - miRNA

KW - microRNA

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DO - 10.1261/rna.646007

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JO - RNA

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A novel monoclonal antibody against human Argonaute proteins reveals unexpected characteristics of miRNAs in human blood cells

Abstract

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Keywords

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