TY - JOUR
T1 - A novel rat model for the study of deficits in bone formation in type-2 diabetes
AU - Liu, Zhendong
AU - Aronson, James
AU - Wahl, Elizabeth C.
AU - Liu, Lichu
AU - Perrien, Daniel S.
AU - Kern, Phillip A.
AU - Fowlkes, John L.
AU - Thrailkill, Kathryn M.
AU - Bunn, Robert C.
AU - Cockrell, Gael E.
AU - Skinner, Robert A.
AU - Lumpkin, Charles K.
PY - 2007/2/1
Y1 - 2007/2/1
N2 - Background: There is evidence to suggest that impairment in bone formation and/or turnover is associated with the metabolic abnormalities characteristic of type2 diabetes mellitus. However, bone regeneration/repair in type-2 diabetes has not been modeled. Using Zucker Diabetic Fatty (ZDF) rats (a model of type-2 diabetes) for tibial distraction osteogenesis (DO), we hypothesized that bone formation within the distraction gap would be impaired. Animals and methods: Rats were examined for body weight, glycosuria, and glycosemia to confirm the diabetic condition during the study. The rats received placement of the external fixators and osteotomies on the left tibia. Distraction was initiated the following day at 0.2 mm twice a day and continued for 14 days. The lengthened tibiae were harvested and distraction gaps were examined radiographically and histologically. Results: We found significant reduction in new bone formation in the distraction gaps of the ZDF rats, both radiographically and histologically, compared to lean rats. We found a decrease in a marker of cellular proliferation in the distraction gaps and increased adipose volume in adjacent bone marrow of the ZDF rats. Interpretation: Our findings suggest that this model might be used to study the contributions of leptin resistance, insulin resistance and/or hyperglycemia to impaired osteoblastogenesis in vivo. Copyright
AB - Background: There is evidence to suggest that impairment in bone formation and/or turnover is associated with the metabolic abnormalities characteristic of type2 diabetes mellitus. However, bone regeneration/repair in type-2 diabetes has not been modeled. Using Zucker Diabetic Fatty (ZDF) rats (a model of type-2 diabetes) for tibial distraction osteogenesis (DO), we hypothesized that bone formation within the distraction gap would be impaired. Animals and methods: Rats were examined for body weight, glycosuria, and glycosemia to confirm the diabetic condition during the study. The rats received placement of the external fixators and osteotomies on the left tibia. Distraction was initiated the following day at 0.2 mm twice a day and continued for 14 days. The lengthened tibiae were harvested and distraction gaps were examined radiographically and histologically. Results: We found significant reduction in new bone formation in the distraction gaps of the ZDF rats, both radiographically and histologically, compared to lean rats. We found a decrease in a marker of cellular proliferation in the distraction gaps and increased adipose volume in adjacent bone marrow of the ZDF rats. Interpretation: Our findings suggest that this model might be used to study the contributions of leptin resistance, insulin resistance and/or hyperglycemia to impaired osteoblastogenesis in vivo. Copyright
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U2 - 10.1080/17453670610013411
DO - 10.1080/17453670610013411
M3 - Article
C2 - 17453392
AN - SCOPUS:34147180478
SN - 1745-3674
VL - 78
SP - 46
EP - 55
JO - Acta Orthopaedica
JF - Acta Orthopaedica
IS - 1
ER -
