TY - JOUR
T1 - A novel regulatory network among LncRpa, CircRar1, MiR-671 and apoptotic genes promotes lead-induced neuronal cell apoptosis
AU - Nan, Aruo
AU - Chen, Lijian
AU - Zhang, Nan
AU - Liu, Zhenzhong
AU - Yang, Ti
AU - Wang, Zhishan
AU - Yang, Chengfeng
AU - Jiang, Yiguo
N1 - Publisher Copyright:
© 2016, The Author(s).
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Lead is a metal that has toxic effects on the developing nervous system. However, the mechanisms underlying lead-induced neurotoxicity are not well understood. Non-coding RNAs (ncRNAs) play an important role in epigenetic regulation, but few studies have examined the function of ncRNAs in lead-induced neurotoxicity. We addressed this in the present study by evaluating the functions of a long non-coding RNA (named lncRpa) and a circular RNA (named circRar1) in a mouse model of lead-induced neurotoxicity. High-throughput RNA sequencing showed that both lncRpa and circRar1 promoted neuronal apoptosis. We also found that lncRpa and circRar1 induced the upregulation of apoptosis-associated factors caspase8 and p38 at the mRNA and protein levels via modulation of their common target microRNA miR-671. This is the first report of a regulatory interaction among a lncRNA, circRNA, and miRNA mediating neuronal apoptosis in response to lead toxicity.
AB - Lead is a metal that has toxic effects on the developing nervous system. However, the mechanisms underlying lead-induced neurotoxicity are not well understood. Non-coding RNAs (ncRNAs) play an important role in epigenetic regulation, but few studies have examined the function of ncRNAs in lead-induced neurotoxicity. We addressed this in the present study by evaluating the functions of a long non-coding RNA (named lncRpa) and a circular RNA (named circRar1) in a mouse model of lead-induced neurotoxicity. High-throughput RNA sequencing showed that both lncRpa and circRar1 promoted neuronal apoptosis. We also found that lncRpa and circRar1 induced the upregulation of apoptosis-associated factors caspase8 and p38 at the mRNA and protein levels via modulation of their common target microRNA miR-671. This is the first report of a regulatory interaction among a lncRNA, circRNA, and miRNA mediating neuronal apoptosis in response to lead toxicity.
KW - Cell apoptosis
KW - CircRNA
KW - Lead
KW - LncRNA
KW - MiRNA
KW - Neurotoxicity
UR - http://www.scopus.com/inward/record.url?scp=84986275862&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84986275862&partnerID=8YFLogxK
U2 - 10.1007/s00204-016-1837-1
DO - 10.1007/s00204-016-1837-1
M3 - Article
C2 - 27604105
AN - SCOPUS:84986275862
SN - 0340-5761
VL - 91
SP - 1671
EP - 1684
JO - Archives of Toxicology
JF - Archives of Toxicology
IS - 4
ER -