A PCR-RFLP for KIT associated with tobiano spotting pattern in horses

S. A. Brooks, R. B. Terry, E. Bailey

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


An MspI polymorphism was identified in intron 13 of the equine homologue of protooncogene c-kit (KIT by comparing DNA sequences from horses with solid coat colour and horses homozygous for the tobiano spotting (To) gene. The allele associated with solid coat colour was designated KMO, while the allele associated with the tobiano pattern created an additional MspI restriction site and was designated KM1. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) studies using DNA from hair follicles demonstrated that all 129 of 129 tobiano patterned horses possessed the KM1 allele. However, three of 104 solid-coloured thoroughbred horses also possessed the KM1 allele. Therefore, while KM1 is strongly associated with the gene for To, the association is not absolute. However, this test appears more efficacious to identify putative homozygotes for To than current biochemical testing methods using albumin (Alb) and vitamin D binding protein (Gc) haplotypes.

Original languageEnglish
Pages (from-to)301-303
Number of pages3
JournalAnimal Genetics
Issue number4
StatePublished - 2002


  • Coat colour
  • Equine
  • KIT
  • Tobiano

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Genetics


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