TY - JOUR
T1 - A pharmacological study of Arabidopsis cell fusion between the persistent synergid and endosperm
AU - Motomura, Kazuki
AU - Kawashima, Tomokazu
AU - Berger, Frédéric
AU - Kinoshita, Tetsu
AU - Higashiyama, Tetsuya
AU - Maruyama, Daisuke
N1 - Publisher Copyright:
© 2018. Published by The Company of Biologists Ltd.
PY - 2018
Y1 - 2018
N2 - Cell fusion is a pivotal process in fertilization and multinucleate cell formation. A plant cell is ubiquitously surrounded by a hard cell wall, and very few cell fusions have been observed except for gamete fusions. We recently reported that the fertilized central cell (the endosperm) absorbs the persistent synergid, a highly differentiated cell necessary for pollen tube attraction. The synergid-endosperm fusion (SE fusion) appears to eliminate the persistent synergid from fertilized ovule in Arabidopsis thaliana. Here, we analyzed the effects of various inhibitors on SE fusion in an in vitro culture system. Different from other cell fusions, neither disruption of actin polymerization nor protein secretion impaired SE fusion. However, transcriptional and translational inhibitors decreased the SE fusion success rate and also inhibited endosperm division. Failures of SE fusion and endosperm nuclear proliferation were also induced by roscovitine, an inhibitor of cyclin-dependent kinases (CDK). These data indicate unique aspects of SE fusion such as independence of filamentous actin support and the importance of CDK-mediated mitotic control.
AB - Cell fusion is a pivotal process in fertilization and multinucleate cell formation. A plant cell is ubiquitously surrounded by a hard cell wall, and very few cell fusions have been observed except for gamete fusions. We recently reported that the fertilized central cell (the endosperm) absorbs the persistent synergid, a highly differentiated cell necessary for pollen tube attraction. The synergid-endosperm fusion (SE fusion) appears to eliminate the persistent synergid from fertilized ovule in Arabidopsis thaliana. Here, we analyzed the effects of various inhibitors on SE fusion in an in vitro culture system. Different from other cell fusions, neither disruption of actin polymerization nor protein secretion impaired SE fusion. However, transcriptional and translational inhibitors decreased the SE fusion success rate and also inhibited endosperm division. Failures of SE fusion and endosperm nuclear proliferation were also induced by roscovitine, an inhibitor of cyclin-dependent kinases (CDK). These data indicate unique aspects of SE fusion such as independence of filamentous actin support and the importance of CDK-mediated mitotic control.
KW - Double fertilization
KW - Endosperm
KW - Pollen tube
KW - Polytubey block
KW - Synergid endosperm fusion
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U2 - 10.1242/jcs.204123
DO - 10.1242/jcs.204123
M3 - Article
C2 - 28808086
AN - SCOPUS:85041216037
SN - 0021-9533
VL - 131
JO - Journal of Cell Science
JF - Journal of Cell Science
IS - 2
M1 - jcs.204123
ER -