TY - JOUR
T1 - A pilot study of estrogen's effects on bronchial myocyte adhesion molecule expression
AU - Dickens, George R.
AU - Matheny, Christopher J.
AU - Morris, Peter E.
AU - Clifton, G. Dennis
AU - Ensom, Mary H.H.
PY - 1999
Y1 - 1999
N2 - We examined the effects of estrogen on tumor necrosis factor α (TNF- α)-induced expression of intracellular adhesion molecule (ICAM-1) and vascular adhesion molecule (VCAM-1) in cultured human bronchial smooth muscle cells (BSMC). Experiments were performed in triplicate in T-75 tissue culture flasks containing normal human BSMC. Four experiments were carried out: untreated BSMC cells (control); TNF-α 1000 U/ml stimulation of BSMC; forskolin 5 μM before TNF-α stimulation of BSMC; and estradiol 30 μM before TNF-α stimulation of BSMC. Cyclic adenosine monophosphate was measured by a commercially available radioimmunoassay kit. Cell expression of ICAM-1 and VCAM-1 was quantified by flow cytometry. Incubation of cells with TNF-α 1000 U/ml for 24 hours elicited a 27-fold increase in basal expression of ICAM-1 and a 2-fold increase in VCAM-1 (p>0.05). Incubation of BSMC with forskolin 5 μM, for 1 hour before TNF-α, decreased TNF-α-induced expression of ICAM-1 by 62% and VCAM-1 slightly by 17%. The BSMC incubated with estradiol 30 μM, 1 hour before TNF-α, decreased TNFα-induced expression of ICAM-1 by 21%; VCAM-1 remained unchanged (p>0.05). We found a trend toward inhibition of TNF-α-stimulated ICAM-1 expression in cultured BSMC with pretreatment with estradiol. However, due to large variability within the cell culture model, statistical significance was not reached.
AB - We examined the effects of estrogen on tumor necrosis factor α (TNF- α)-induced expression of intracellular adhesion molecule (ICAM-1) and vascular adhesion molecule (VCAM-1) in cultured human bronchial smooth muscle cells (BSMC). Experiments were performed in triplicate in T-75 tissue culture flasks containing normal human BSMC. Four experiments were carried out: untreated BSMC cells (control); TNF-α 1000 U/ml stimulation of BSMC; forskolin 5 μM before TNF-α stimulation of BSMC; and estradiol 30 μM before TNF-α stimulation of BSMC. Cyclic adenosine monophosphate was measured by a commercially available radioimmunoassay kit. Cell expression of ICAM-1 and VCAM-1 was quantified by flow cytometry. Incubation of cells with TNF-α 1000 U/ml for 24 hours elicited a 27-fold increase in basal expression of ICAM-1 and a 2-fold increase in VCAM-1 (p>0.05). Incubation of BSMC with forskolin 5 μM, for 1 hour before TNF-α, decreased TNF-α-induced expression of ICAM-1 by 62% and VCAM-1 slightly by 17%. The BSMC incubated with estradiol 30 μM, 1 hour before TNF-α, decreased TNFα-induced expression of ICAM-1 by 21%; VCAM-1 remained unchanged (p>0.05). We found a trend toward inhibition of TNF-α-stimulated ICAM-1 expression in cultured BSMC with pretreatment with estradiol. However, due to large variability within the cell culture model, statistical significance was not reached.
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U2 - 10.1592/phco.19.18.1426.30902
DO - 10.1592/phco.19.18.1426.30902
M3 - Article
C2 - 10600091
AN - SCOPUS:0032713239
VL - 19
SP - 1426
EP - 1431
IS - 12
ER -