A quinoline fluorescence method for visualizing and assaying the histochemically reactive zinc (bouton zinc) in the brain

C. J. Frederickson, E. J. Kasarskis, D. Ringo, R. E. Frederickson

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518 Scopus citations

Abstract

A histochemical method for staining CNS zinc by the stoichiometric formation of zinc: quinoline fluorescent chelates is described. Four congeners of quinoline have been tested, and two found to be useful for histochemistry. The procedure is a one-step staining method, suitable for fresh-frozen and fixed tissue sections alike. The quinoline fluorescence selectively labels the CNS regions (such as hippocampus, amygdala) shown by prior histochemical procedures to be rich in histochemically reactive zinc in axon boutons and therefore appears to be a specific marker for the bouton zinc. Microfluorometric data indicate that the fluorochrome can be used for quantitative estimates of CNS zinc pools as well as qualitative studies of localization.

Original languageEnglish
Pages (from-to)91-103
Number of pages13
JournalJournal of Neuroscience Methods
Volume20
Issue number2
DOIs
StatePublished - Jun 1987

Bibliographical note

Funding Information:
Supported in part by the Veteran's Administration Research Service and Grants NS 00768 and AA 05931-02 (E.J.K.) and R03 MH41691-01 and NIH/BRSG 807-RR07133 (C.J.F.).

Keywords

  • Axon bouton
  • Synapse
  • Vesicle
  • Zinc-containing neuron

ASJC Scopus subject areas

  • General Neuroscience

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