TY - JOUR
T1 - A rapid, non-radioactive method for the detection of insulin-like growth factor binding proteins by Western ligand blotting
AU - Fowlkes, John L.
AU - Serra, Delila
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 1996
Y1 - 1996
N2 - Insulin-like growth factor binding proteins (IGFBPs) are produced by many cell types and are present in a wide variety of biological fluids. Detection of IGFBPs in complex fluids is commonly performed by Western ligand blotting using 125I-IGF-I and/or 125I-IGF-II as the labeling ligand. This method is extremely useful in detecting IGFBPs; however, it requires the use of radioactive materials and usually takes several days to weeks to complete. Herein, we report on a modification of this method using biotinylated IGFs to detect both purified recombinant IGFBPs, as well as IGFBPs in fibroblast conditioned medium. This alternative method has proved sensitive, time- and cost-efficient, and it is environmentally safe.
AB - Insulin-like growth factor binding proteins (IGFBPs) are produced by many cell types and are present in a wide variety of biological fluids. Detection of IGFBPs in complex fluids is commonly performed by Western ligand blotting using 125I-IGF-I and/or 125I-IGF-II as the labeling ligand. This method is extremely useful in detecting IGFBPs; however, it requires the use of radioactive materials and usually takes several days to weeks to complete. Herein, we report on a modification of this method using biotinylated IGFs to detect both purified recombinant IGFBPs, as well as IGFBPs in fibroblast conditioned medium. This alternative method has proved sensitive, time- and cost-efficient, and it is environmentally safe.
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U2 - 10.1210/endo.137.12.8940413
DO - 10.1210/endo.137.12.8940413
M3 - Article
C2 - 8940413
AN - SCOPUS:0030471914
SN - 0013-7227
VL - 137
SP - 5751
EP - 5754
JO - Endocrinology
JF - Endocrinology
IS - 12
ER -