A rapid, simple, and inexpensive method for the preparation of strand-specific RNA-Seq libraries

Research output: Contribution to journalArticlepeer-review

13 Scopus citations

Abstract

High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNApoly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specifi c RNA-Seq libraries from varying quantities of total RNA.

Original languageEnglish
Pages (from-to)195-207
Number of pages13
JournalMethods in Molecular Biology
Volume1255
DOIs
StatePublished - 2015

Bibliographical note

Publisher Copyright:
© Springer Science+Business Media New York 2015.

Keywords

  • Alternative polyadenylation
  • Gene expression
  • High-throughput sequencing
  • Strandspecific RNA-Seq

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology

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