Abstract
High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNApoly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specifi c RNA-Seq libraries from varying quantities of total RNA.
Original language | English |
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Pages (from-to) | 195-207 |
Number of pages | 13 |
Journal | Methods in Molecular Biology |
Volume | 1255 |
DOIs | |
State | Published - 2015 |
Bibliographical note
Publisher Copyright:© Springer Science+Business Media New York 2015.
Keywords
- Alternative polyadenylation
- Gene expression
- High-throughput sequencing
- Strandspecific RNA-Seq
ASJC Scopus subject areas
- Genetics
- Molecular Biology