TY - JOUR
T1 - A rapid, simple, and inexpensive method for the preparation of strand-specific RNA-Seq libraries
AU - Hunt, Arthur G.
PY - 2015
Y1 - 2015
N2 - High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNA-poly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specific RNA-Seq libraries from varying quantities of total RNA.
AB - High-throughput sequencing of short cDNA tags, or RNA-Seq, has become a staple of genome-wide gene expression studies in plants. RNA-Seq libraries necessarily contain tags that correspond to the mRNA-poly(A) junction, or polyadenylation site, and thus may be mined for data that can help study alternative polyadenylation. This report presents a simple, rapid, and inexpensive method for preparing strand-specific RNA-Seq libraries from varying quantities of total RNA.
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UR - http://www.scopus.com/inward/citedby.url?scp=84954622548&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2175-1_17
DO - 10.1007/978-1-4939-2175-1_17
M3 - Article
C2 - 25487215
AN - SCOPUS:84954622548
VL - 1255
SP - 195
EP - 207
ER -