A real-time polymerase chain reaction protocol for quantifying growth of Fusarium graminearum during solid substrate cultivation on corn stover

Research output: Contribution to journalArticlepeer-review

Abstract

Solid substrate cultivation (SSC), in which microbes are grown on solid substrates in the absence of free water, is a traditional technology with the potential to produce novel chemicals and biomass products for use in the manufacture of biofuels. It is currently relatively difficult to accurately measure microbial growth on a solid substrate, and thus to optimize SSC production conditions. A quantitative real-time polymerase-chain reaction protocol (RT-PCR) was developed to measure growth of the corn stalk rot fungus Fusarium graminearum on corn stover. The RT-PCR assay gave results that were comparable to more traditional glucosamine assays, but it was more sensitive. Other advantages of the RT-PCR assay include its specificity and the potential for high-throughput automation. The RT-PCR approach will facilitate optimization of culture conditions in SSC systems, thus increasing the potential for using SSC in the future for chemical production and biomass processing.

Original languageEnglish
Pages (from-to)144-155
Number of pages12
JournalJournal of Biotech Research
Volume2
Issue number1
StatePublished - 2010

ASJC Scopus subject areas

  • Biotechnology
  • Renewable Energy, Sustainability and the Environment
  • Genetics

Fingerprint

Dive into the research topics of 'A real-time polymerase chain reaction protocol for quantifying growth of Fusarium graminearum during solid substrate cultivation on corn stover'. Together they form a unique fingerprint.

Cite this