A response to Gupta et al. (2019) regarding the MOT3 wheat blast diagnostic assay

Jarred Yasuhara-Bell; Michael L. Pieck; Amy Ruck; Mark L. Farman; Gary L. Peterson; James P. Stack; Barbara Valent; Kerry F. Pedley

doi:10.1094/PHYTO-10-18-0397-LE

A response to Gupta et al. (2019) regarding the MOT3 wheat blast diagnostic assay

Jarred Yasuhara-Bell, Michael L. Pieck, Amy Ruck, Mark L. Farman, Gary L. Peterson, James P. Stack, Barbara Valent, Kerry F. Pedley

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

This is a response to a recent Letter to the Editor of Phytopathology, in which Gupta et al. (2019) caution against the indiscriminate use of the MoT3 diagnostic assay that distinguishes isolates of Magnaporthe oryzae in the Triticum lineage from those that do not cause aggressive wheat blast. We confirm that the assay does reliably distinguish between wheat and rice isolates from Bangladesh and worldwide, as described in the original paper by Pieck et al. (2017). We have been unable to reproduce the equally intense amplification of WB12 and WB12-like sequences reported in Figure 1 of the Letter. Other data presented by Gupta et al. (2019) support the specificity of the MoT3 assay. Therefore, cautions beyond those always associated with accurate reproduction of diagnostic assays are unwarranted.

Original language	English
Pages (from-to)	509-511
Number of pages	3
Journal	Phytopathology
Volume	109
Issue number	4
DOIs	https://doi.org/10.1094/PHYTO-10-18-0397-LE
State	Published - Apr 2019

Bibliographical note

Publisher Copyright:
© 2019 American Phytopathological Society. All rights reserved.

Funding

Funding: This project is supported by the USDA-National Institute of Food and Agriculture, Agriculture and Food Research Initiative Competitive Grant number 2013-68004-20378 and by the USDA-Agricultural Research Service Project number 8044-22000-046-00D. Contribution no. 19-079-J from the Kansas Agricultural Experiment Station. We thank T. Islam, S. Kamoun, and N. Talbot for fruitful discussions and for providing Bangladeshi rice isolates used in their study. Funding: This project is supported by the USDA-National Institute of Food and Agriculture, Agriculture and Food Research Initiative Competitive Grant number 2013-68004-20378 and by the USDA-Agricultural Research Service Project number 8044-22000-046-00D. Contribution no. 19-079-J from the Kansas Agricultural Experiment Station.

Funders	Funder number
USDA-Agricultural Research Service
USDA National Institute of Food and Agriculture	2013-68004-20378
USDA-Agricultural Research Service	8044-22000-046-00D

ASJC Scopus subject areas

Agronomy and Crop Science
Plant Science

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10.1094/PHYTO-10-18-0397-LE

Cite this

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abstract = "This is a response to a recent Letter to the Editor of Phytopathology, in which Gupta et al. (2019) caution against the indiscriminate use of the MoT3 diagnostic assay that distinguishes isolates of Magnaporthe oryzae in the Triticum lineage from those that do not cause aggressive wheat blast. We confirm that the assay does reliably distinguish between wheat and rice isolates from Bangladesh and worldwide, as described in the original paper by Pieck et al. (2017). We have been unable to reproduce the equally intense amplification of WB12 and WB12-like sequences reported in Figure 1 of the Letter. Other data presented by Gupta et al. (2019) support the specificity of the MoT3 assay. Therefore, cautions beyond those always associated with accurate reproduction of diagnostic assays are unwarranted.",

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AU - Peterson, Gary L.

AU - Stack, James P.

AU - Valent, Barbara

AU - Pedley, Kerry F.

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A response to Gupta et al. (2019) regarding the MOT3 wheat blast diagnostic assay

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

Access to Document

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