A shared cis-regulatory module activates transcription in the suspensor of plant embryos

Kelli F. Henry, Anhthu Q. Bui, Tomokazu Kawashima, Robert B. Goldberg

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


The mechanisms controlling the transcription of gene sets in specific regions of a plant embryo shortly after fertilization remain unknown. Previously, we showed that G564 mRNA, encoding a protein of unknown function, accumulates to high levels in the giant suspensor of both Scarlet Runner Bean (SRB) and Common Bean embryos, and a cis-regulatory module containing three unique DNA sequences, designated as the 10-bp, Region 2, and Fifth motifs, is required for G564 suspensor-specific transcription [Henry KF, et al. (2015) Plant Mol Biol 88:207-217; Kawashima T, et al. (2009) Proc Natl Acad Sci USA 106:3627-3632]. We tested the hypothesis that these motifs are also required for transcription of the SRB GA 20-oxidase gene, which encodes a gibberellic acid hormone biosynthesis enzyme and is co-expressed with G564 at a high level in giant bean suspensors. We used deletion and gain-of-function experiments in transgenic tobacco embryos to show that two GA 20-oxidase DNA regions are required for suspensor-specific transcription, one in the 5′ UTR (+119 to +205) and another in the 5′ upstream region (−341 to −316). Mutagenesis of sequences in these two regions determined that the cis-regulatory motifs required for G564 suspensor transcription are also required for GA 20-oxidase transcription within the suspensor, although the motif arrangement differs. Our results demonstrate the flexibility of motif positioning within a cis-regulatory module that activates gene transcription within giant bean suspensors and suggest that G564 and GA 20-oxidase comprise part of a suspensor gene regulatory network.

Original languageEnglish
Pages (from-to)E5824-E5833
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number25
StatePublished - Jun 19 2018

Bibliographical note

Publisher Copyright:
© 2018 National Academy of Sciences. All Rights Reserved.


  • Cis-regulatory modules
  • Plant embryos
  • Promoter analysis
  • Scarlet Runner Bean
  • Suspensor

ASJC Scopus subject areas

  • General


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