A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization

Joseph LeSauter; Lily Yan; Bhavana Vishnubhotla; Jorge E. Quintero; Sandra J. Kuhlman; Douglas G. McMahon; Rae Silver

doi:10.1016/S0006-8993(02)04084-2

A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization

Joseph LeSauter, Lily Yan, Bhavana Vishnubhotla, Jorge E. Quintero, Sandra J. Kuhlman, Douglas G. McMahon, Rae Silver

Neurosurgery

Research output: Contribution to journal › Article › peer-review

52 Scopus citations

Abstract

Period1 (Per1) is one of several clock genes driving the oscillatory mechanisms that mediate circadian rhythmicity. Per1 mRNA and protein are highly expressed in the suprachiasmatic nuclei, which contain oscillator cells that drive circadian rhythmicity in physiological and behavioral responses. We examined a transgenic mouse in which degradable green fluorescent protein (GFP) is driven by the mPer1 gene promoter. This mouse expresses precise free-running rhythms and characteristic light induced phase shifts. GFP protein (reporting Per1 mRNA) is expressed rhythmically as measured by either fluorescence or immunocytochemistry. In addition the animals show predicted rhythms of Per1 mRNA, PER1 and PER2 proteins. The localization of GFP overlaps with that of Per1 mRNA, PER1 and PER2 proteins. Together, these results suggest that GFP reports rhythmic Per1 expression. A surprising finding is that, at their peak expression time GFP, Per1 mRNA, PER1 and PER2 proteins are absent or not detectable in a subpopulation of SCN cells located in the core region of the nucleus.

Original language	English
Pages (from-to)	279-287
Number of pages	9
Journal	Brain Research
Volume	964
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(02)04084-2
State	Published - Feb 28 2003

Bibliographical note

Funding Information:
We are grateful to Dr Michael Lehman for his advice on this manuscript. This study was supported by NIH Grants NS-37919 to RS, MH63341 and EY09256 to DGM.

Keywords

Circadian rhythm
Green fluorescent protein
Period gene
Suprachiasmatic nucleus
Transgenic

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(02)04084-2

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title = "A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization",

abstract = "Period1 (Per1) is one of several clock genes driving the oscillatory mechanisms that mediate circadian rhythmicity. Per1 mRNA and protein are highly expressed in the suprachiasmatic nuclei, which contain oscillator cells that drive circadian rhythmicity in physiological and behavioral responses. We examined a transgenic mouse in which degradable green fluorescent protein (GFP) is driven by the mPer1 gene promoter. This mouse expresses precise free-running rhythms and characteristic light induced phase shifts. GFP protein (reporting Per1 mRNA) is expressed rhythmically as measured by either fluorescence or immunocytochemistry. In addition the animals show predicted rhythms of Per1 mRNA, PER1 and PER2 proteins. The localization of GFP overlaps with that of Per1 mRNA, PER1 and PER2 proteins. Together, these results suggest that GFP reports rhythmic Per1 expression. A surprising finding is that, at their peak expression time GFP, Per1 mRNA, PER1 and PER2 proteins are absent or not detectable in a subpopulation of SCN cells located in the core region of the nucleus.",

keywords = "Circadian rhythm, Green fluorescent protein, Period gene, Suprachiasmatic nucleus, Transgenic",

author = "Joseph LeSauter and Lily Yan and Bhavana Vishnubhotla and Quintero, {Jorge E.} and Kuhlman, {Sandra J.} and McMahon, {Douglas G.} and Rae Silver",

note = "Funding Information: We are grateful to Dr Michael Lehman for his advice on this manuscript. This study was supported by NIH Grants NS-37919 to RS, MH63341 and EY09256 to DGM.",

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AU - LeSauter, Joseph

AU - Yan, Lily

AU - Vishnubhotla, Bhavana

AU - Quintero, Jorge E.

AU - Kuhlman, Sandra J.

AU - McMahon, Douglas G.

AU - Silver, Rae

N1 - Funding Information: We are grateful to Dr Michael Lehman for his advice on this manuscript. This study was supported by NIH Grants NS-37919 to RS, MH63341 and EY09256 to DGM.

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N2 - Period1 (Per1) is one of several clock genes driving the oscillatory mechanisms that mediate circadian rhythmicity. Per1 mRNA and protein are highly expressed in the suprachiasmatic nuclei, which contain oscillator cells that drive circadian rhythmicity in physiological and behavioral responses. We examined a transgenic mouse in which degradable green fluorescent protein (GFP) is driven by the mPer1 gene promoter. This mouse expresses precise free-running rhythms and characteristic light induced phase shifts. GFP protein (reporting Per1 mRNA) is expressed rhythmically as measured by either fluorescence or immunocytochemistry. In addition the animals show predicted rhythms of Per1 mRNA, PER1 and PER2 proteins. The localization of GFP overlaps with that of Per1 mRNA, PER1 and PER2 proteins. Together, these results suggest that GFP reports rhythmic Per1 expression. A surprising finding is that, at their peak expression time GFP, Per1 mRNA, PER1 and PER2 proteins are absent or not detectable in a subpopulation of SCN cells located in the core region of the nucleus.

AB - Period1 (Per1) is one of several clock genes driving the oscillatory mechanisms that mediate circadian rhythmicity. Per1 mRNA and protein are highly expressed in the suprachiasmatic nuclei, which contain oscillator cells that drive circadian rhythmicity in physiological and behavioral responses. We examined a transgenic mouse in which degradable green fluorescent protein (GFP) is driven by the mPer1 gene promoter. This mouse expresses precise free-running rhythms and characteristic light induced phase shifts. GFP protein (reporting Per1 mRNA) is expressed rhythmically as measured by either fluorescence or immunocytochemistry. In addition the animals show predicted rhythms of Per1 mRNA, PER1 and PER2 proteins. The localization of GFP overlaps with that of Per1 mRNA, PER1 and PER2 proteins. Together, these results suggest that GFP reports rhythmic Per1 expression. A surprising finding is that, at their peak expression time GFP, Per1 mRNA, PER1 and PER2 proteins are absent or not detectable in a subpopulation of SCN cells located in the core region of the nucleus.

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KW - Period gene

KW - Suprachiasmatic nucleus

KW - Transgenic

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A short half-life GFP mouse model for analysis of suprachiasmatic nucleus organization

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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