A single-step reverse transcription loop-mediated isothermal amplification assay for rapid and accurate detection of Pepper vein yellows virus

Mengting Jiang, Xiangwen Luo, Deyong Zhang, Xuguo Zhou, Xian Ouyang, Zhanhong Zhang, Fan Li, Songbai Zhang, Yong Liu

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Pepper vein yellows virus (PeVYV) is a member of Polerovirus and infects solanaceous crops worldwide. In this study, a highly efficient and easy-to-use single-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for PeVYV detection. This assay used two outer (F3 and B3), two inner (FIP and BIP) and two loop (LF and LB) primers in a single reaction. The optimized reaction was determined to be at 62°C for 45 min and the whole reaction took less than 1 h. This assay was about 10 times more sensitive than the conventional one-step RT-PCR. This RT-LAMP assay should allow specific detection of PeVYV in plant tissues. We recommend this PeVYV-specific RT-LAMP assay for large scale field studies of PeVYV infection.

Original languageEnglish
Pages (from-to)212-217
Number of pages6
JournalCanadian Journal of Plant Pathology
Volume39
Issue number2
DOIs
StatePublished - Apr 3 2017

Bibliographical note

Publisher Copyright:
© 2017 Hunan Plant Protection Institute.

Keywords

  • PeVYV
  • Pepper vein yellows virus
  • RT-LAMP
  • RT-PCR
  • detection
  • pepper

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Plant Science

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