Abstract
Pepper vein yellows virus (PeVYV) is a member of Polerovirus and infects solanaceous crops worldwide. In this study, a highly efficient and easy-to-use single-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for PeVYV detection. This assay used two outer (F3 and B3), two inner (FIP and BIP) and two loop (LF and LB) primers in a single reaction. The optimized reaction was determined to be at 62°C for 45 min and the whole reaction took less than 1 h. This assay was about 10 times more sensitive than the conventional one-step RT-PCR. This RT-LAMP assay should allow specific detection of PeVYV in plant tissues. We recommend this PeVYV-specific RT-LAMP assay for large scale field studies of PeVYV infection.
| Original language | English |
|---|---|
| Pages (from-to) | 212-217 |
| Number of pages | 6 |
| Journal | Canadian Journal of Plant Pathology |
| Volume | 39 |
| Issue number | 2 |
| DOIs | |
| State | Published - Apr 3 2017 |
Bibliographical note
Publisher Copyright:© 2017 Hunan Plant Protection Institute.
Funding
This work was supported by the National Natural Science Foundation of China [31571982]; Agriculture Research System of China [CARS-25-B-05]; the Special Fund for Agro-scientific Research in the Public Interest [201303028].
| Funders | Funder number |
|---|---|
| National Natural Science Foundation of China (NSFC) | 31571982 |
| Special Fund for Agro-scientific Research in the Public Interest | 201303028 |
| Agriculture Research System of China | CARS-25-B-05 |
Keywords
- PeVYV
- Pepper vein yellows virus
- RT-LAMP
- RT-PCR
- detection
- pepper
ASJC Scopus subject areas
- Agronomy and Crop Science
- Plant Science