TY - JOUR
T1 - A single-step reverse transcription loop-mediated isothermal amplification assay for rapid and accurate detection of Pepper vein yellows virus
AU - Jiang, Mengting
AU - Luo, Xiangwen
AU - Zhang, Deyong
AU - Zhou, Xuguo
AU - Ouyang, Xian
AU - Zhang, Zhanhong
AU - Li, Fan
AU - Zhang, Songbai
AU - Liu, Yong
N1 - Publisher Copyright:
© 2017 Hunan Plant Protection Institute.
PY - 2017/4/3
Y1 - 2017/4/3
N2 - Pepper vein yellows virus (PeVYV) is a member of Polerovirus and infects solanaceous crops worldwide. In this study, a highly efficient and easy-to-use single-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for PeVYV detection. This assay used two outer (F3 and B3), two inner (FIP and BIP) and two loop (LF and LB) primers in a single reaction. The optimized reaction was determined to be at 62°C for 45 min and the whole reaction took less than 1 h. This assay was about 10 times more sensitive than the conventional one-step RT-PCR. This RT-LAMP assay should allow specific detection of PeVYV in plant tissues. We recommend this PeVYV-specific RT-LAMP assay for large scale field studies of PeVYV infection.
AB - Pepper vein yellows virus (PeVYV) is a member of Polerovirus and infects solanaceous crops worldwide. In this study, a highly efficient and easy-to-use single-step reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for PeVYV detection. This assay used two outer (F3 and B3), two inner (FIP and BIP) and two loop (LF and LB) primers in a single reaction. The optimized reaction was determined to be at 62°C for 45 min and the whole reaction took less than 1 h. This assay was about 10 times more sensitive than the conventional one-step RT-PCR. This RT-LAMP assay should allow specific detection of PeVYV in plant tissues. We recommend this PeVYV-specific RT-LAMP assay for large scale field studies of PeVYV infection.
KW - PeVYV
KW - Pepper vein yellows virus
KW - RT-LAMP
KW - RT-PCR
KW - detection
KW - pepper
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U2 - 10.1080/07060661.2017.1316774
DO - 10.1080/07060661.2017.1316774
M3 - Article
AN - SCOPUS:85019650733
SN - 0706-0661
VL - 39
SP - 212
EP - 217
JO - Canadian Journal of Plant Pathology
JF - Canadian Journal of Plant Pathology
IS - 2
ER -