TY - JOUR
T1 - A slot blot method for detection of ultraviolet photoproducts in DNA
AU - Liu, Xiaoqi
AU - Smerdon, Michael J.
PY - 1995/8
Y1 - 1995/8
N2 - A simple new method was established to detect the major uv photoproducts in DNA. A slot blot method, involving the use of T4 DNA polymerase-associated (3′ → 5′) exonuclease digestion of uv-irradiated DNA, was used to detect (primarily) cyclobutane pyrimidine dimers and pyrimidine-pyrimidone (6-4) dimers. Hydrolysis of DNA by this enzyme is quantitatively blocked by both of these photoproducts. To detect (primarily) (6-4) dimers, Escherichia coli DNA photolyase was utilized to specifically reverse cyclobutane pyrimidine dimers.
AB - A simple new method was established to detect the major uv photoproducts in DNA. A slot blot method, involving the use of T4 DNA polymerase-associated (3′ → 5′) exonuclease digestion of uv-irradiated DNA, was used to detect (primarily) cyclobutane pyrimidine dimers and pyrimidine-pyrimidone (6-4) dimers. Hydrolysis of DNA by this enzyme is quantitatively blocked by both of these photoproducts. To detect (primarily) (6-4) dimers, Escherichia coli DNA photolyase was utilized to specifically reverse cyclobutane pyrimidine dimers.
UR - http://www.scopus.com/inward/record.url?scp=0029077287&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0029077287&partnerID=8YFLogxK
U2 - 10.1006/abio.1995.1420
DO - 10.1006/abio.1995.1420
M3 - Article
C2 - 7485990
AN - SCOPUS:0029077287
SN - 0003-2697
VL - 229
SP - 323
EP - 328
JO - Analytical Biochemistry
JF - Analytical Biochemistry
IS - 2
ER -