A specialist herbivore pest adaptation to xenobiotics through up-regulation of multiple Cytochrome P450s

Fang Zhu; Timothy W. Moural; David R. Nelson; Subba R. Palli

doi:10.1038/srep20421

A specialist herbivore pest adaptation to xenobiotics through up-regulation of multiple Cytochrome P450s

Fang Zhu, Timothy W. Moural, David R. Nelson, Subba R. Palli

Research output: Contribution to journal › Article › peer-review

97 Scopus citations

Abstract

The adaptation of herbivorous insects to their host plants is hypothesized to be intimately associated with their ubiquitous development of resistance to synthetic pesticides. However, not much is known about the mechanisms underlying the relationship between detoxification of plant toxins and synthetic pesticides. To address this knowledge gap, we used specialist pest Colorado potato beetle (CPB) and its host plant, potato, as a model system. Next-generation sequencing (454 pyrosequencing) was performed to reveal the CPB transcriptome. Differential expression patterns of cytochrome P450 complement (CYPome) were analyzed between the susceptible (S) and imidacloprid resistant (R) beetles. We also evaluated the global transcriptome repertoire of CPB CYPome in response to the challenge by potato leaf allelochemicals and imidacloprid. The results showed that more than half (51.2%) of the CBP cytochrome P450 monooxygenases (P450s) that are up-regulated in the R strain are also induced by both host plant toxins and pesticide in a tissue-specific manner. These data suggest that xenobiotic adaptation in this specialist herbivore is through up-regulation of multiple P450s that are potentially involved in detoxifying both pesticide and plant allelochemicals.

Original language	English
Article number	20421
Journal	Scientific Reports
Volume	6
DOIs	https://doi.org/10.1038/srep20421
State	Published - Feb 10 2016

Bibliographical note

Publisher Copyright:
© 2016, Nature Publishing Group. All rights reserved.

Funding

The authors are grateful to Andrei Alyokhin (University of Maine), Kathleen Schnaars-Uvino (Queens College), and Sandy Menasha (Cornell Cooperative Extension) for help with collection of CPB imidacloprid resistant beetles. We thank John Wigginton, Hemant Gujar, Jingjing Xu and Yingjun Cui (University of Kentucky) for their technical assistance. This is publication number 16-08-009 from the Kentucky Agricultural Experimental Station and published with the approval of the director. This work is supported by USDA-NRI-CSREES (2011-04636) and HATCH under 2351177000.

Funders	Funder number
HATCH	2351177000
NRI/CSREES/USDA	2011-04636

ASJC Scopus subject areas

General

Access to Document

10.1038/srep20421

Cite this

@article{4d2fa86b6eb342ed86947715f391f052,

title = "A specialist herbivore pest adaptation to xenobiotics through up-regulation of multiple Cytochrome P450s",

abstract = "The adaptation of herbivorous insects to their host plants is hypothesized to be intimately associated with their ubiquitous development of resistance to synthetic pesticides. However, not much is known about the mechanisms underlying the relationship between detoxification of plant toxins and synthetic pesticides. To address this knowledge gap, we used specialist pest Colorado potato beetle (CPB) and its host plant, potato, as a model system. Next-generation sequencing (454 pyrosequencing) was performed to reveal the CPB transcriptome. Differential expression patterns of cytochrome P450 complement (CYPome) were analyzed between the susceptible (S) and imidacloprid resistant (R) beetles. We also evaluated the global transcriptome repertoire of CPB CYPome in response to the challenge by potato leaf allelochemicals and imidacloprid. The results showed that more than half (51.2%) of the CBP cytochrome P450 monooxygenases (P450s) that are up-regulated in the R strain are also induced by both host plant toxins and pesticide in a tissue-specific manner. These data suggest that xenobiotic adaptation in this specialist herbivore is through up-regulation of multiple P450s that are potentially involved in detoxifying both pesticide and plant allelochemicals.",

author = "Fang Zhu and Moural, {Timothy W.} and Nelson, {David R.} and Palli, {Subba R.}",

year = "2016",

month = feb,

day = "10",

doi = "10.1038/srep20421",

language = "English",

volume = "6",

}

TY - JOUR

T1 - A specialist herbivore pest adaptation to xenobiotics through up-regulation of multiple Cytochrome P450s

AU - Zhu, Fang

AU - Moural, Timothy W.

AU - Nelson, David R.

AU - Palli, Subba R.

PY - 2016/2/10

Y1 - 2016/2/10

N2 - The adaptation of herbivorous insects to their host plants is hypothesized to be intimately associated with their ubiquitous development of resistance to synthetic pesticides. However, not much is known about the mechanisms underlying the relationship between detoxification of plant toxins and synthetic pesticides. To address this knowledge gap, we used specialist pest Colorado potato beetle (CPB) and its host plant, potato, as a model system. Next-generation sequencing (454 pyrosequencing) was performed to reveal the CPB transcriptome. Differential expression patterns of cytochrome P450 complement (CYPome) were analyzed between the susceptible (S) and imidacloprid resistant (R) beetles. We also evaluated the global transcriptome repertoire of CPB CYPome in response to the challenge by potato leaf allelochemicals and imidacloprid. The results showed that more than half (51.2%) of the CBP cytochrome P450 monooxygenases (P450s) that are up-regulated in the R strain are also induced by both host plant toxins and pesticide in a tissue-specific manner. These data suggest that xenobiotic adaptation in this specialist herbivore is through up-regulation of multiple P450s that are potentially involved in detoxifying both pesticide and plant allelochemicals.

AB - The adaptation of herbivorous insects to their host plants is hypothesized to be intimately associated with their ubiquitous development of resistance to synthetic pesticides. However, not much is known about the mechanisms underlying the relationship between detoxification of plant toxins and synthetic pesticides. To address this knowledge gap, we used specialist pest Colorado potato beetle (CPB) and its host plant, potato, as a model system. Next-generation sequencing (454 pyrosequencing) was performed to reveal the CPB transcriptome. Differential expression patterns of cytochrome P450 complement (CYPome) were analyzed between the susceptible (S) and imidacloprid resistant (R) beetles. We also evaluated the global transcriptome repertoire of CPB CYPome in response to the challenge by potato leaf allelochemicals and imidacloprid. The results showed that more than half (51.2%) of the CBP cytochrome P450 monooxygenases (P450s) that are up-regulated in the R strain are also induced by both host plant toxins and pesticide in a tissue-specific manner. These data suggest that xenobiotic adaptation in this specialist herbivore is through up-regulation of multiple P450s that are potentially involved in detoxifying both pesticide and plant allelochemicals.

UR - http://www.scopus.com/inward/record.url?scp=84957568508&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84957568508&partnerID=8YFLogxK

U2 - 10.1038/srep20421

DO - 10.1038/srep20421

M3 - Article

C2 - 26861263

AN - SCOPUS:84957568508

SN - 2045-2322

VL - 6

JO - Scientific Reports

JF - Scientific Reports

M1 - 20421

ER -

A specialist herbivore pest adaptation to xenobiotics through up-regulation of multiple Cytochrome P450s

Abstract

Bibliographical note

Funding

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this