B lymphocytes from aged mice were found to be defective in their ability to proliferate in response to stimulation with an autoreactive T cell clone D1.4. The differentiative response leading to antibody secretion was also impaired in the auto D1.4 T cell-stimulated B cells from old mice in comparison to similarly stimulated B cells from young mice. The B cells from old mice were competent in activating the autoreactive T cells such that the T cells were induced to proliferate. The B cell defect appears to be restricted to a certain phase of B cell activation, since old mouse B cells responded to the auto D1.4 T cells by increasing cell surface Ia as well as size, but failed to incorporate tritiated thymidine. The responsiveness to interleukin-4 was found to be similar between B cells from young and old mice. It appeared that the B cells from old mice are specifically defective in progressing from the g0 phase of cell cycle into the S phase when stimulated with the auto D1.4 T cells.
|Number of pages||12|
|State||Published - Apr 15 1989|
Bibliographical noteFunding Information:
’ Supported in part by NIH Grants CA 45009 and CA45010 to P.S.N. and M.N., AI 21490, AG-05731, and an appropriation from the PSP funds of University of Kentucky to B.S. B.S. is a recipient of Research Career Development Award 1K 04AGOO422 from the NIH. ’ To whom all correspondence should be addresseda t The Sanders Brown Center on Aging, University of Kentucky, Lexington, KY 405360230. 3 Abbreviations used: Ig, immunoglobulin; SMLR, syngeneic mixed lymphocyte reaction; IL, interleukin; ELISA, enzyme-linked immunosorbent assay.
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