A strategy for dissecting the architectures of native macromolecular assemblies

Yi Shi, Riccardo Pellarin, Peter C. Fridy, Javier Fernandez-Martinez, Mary K. Thompson, Yinyin Li, Qing Jun Wang, Andrej Sali, Michael P. Rout, Brian T. Chait

Research output: Contribution to journalArticlepeer-review

94 Scopus citations


It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.

Original languageEnglish
Pages (from-to)1135-1138
Number of pages4
JournalNature Methods
Issue number12
StatePublished - Dec 1 2015

Bibliographical note

Publisher Copyright:
© 2015 Nature America, Inc.

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology


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