A strategy for dissecting the architectures of native macromolecular assemblies

Yi Shi; Riccardo Pellarin; Peter C. Fridy; Javier Fernandez-Martinez; Mary K. Thompson; Yinyin Li; Qing Jun Wang; Andrej Sali; Michael P. Rout; Brian T. Chait

doi:10.1038/nmeth.3617

A strategy for dissecting the architectures of native macromolecular assemblies

Yi Shi, Riccardo Pellarin, Peter C. Fridy, Javier Fernandez-Martinez, Mary K. Thompson, Yinyin Li, Qing Jun Wang, Andrej Sali, Michael P. Rout, Brian T. Chait

Opthalmology

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.

Original language	English
Pages (from-to)	1135-1138
Number of pages	4
Journal	Nature Methods
Volume	12
Issue number	12
DOIs	https://doi.org/10.1038/nmeth.3617
State	Published - Dec 1 2015

Bibliographical note

Publisher Copyright:
© 2015 Nature America, Inc.

ASJC Scopus subject areas

Biotechnology
Biochemistry
Molecular Biology
Cell Biology

Access to Document

10.1038/nmeth.3617

Cite this

@article{c541dcdc87ab42c08e528df5b0f8d9c7,

title = "A strategy for dissecting the architectures of native macromolecular assemblies",

abstract = "It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.",

author = "Yi Shi and Riccardo Pellarin and Fridy, {Peter C.} and Javier Fernandez-Martinez and Thompson, {Mary K.} and Yinyin Li and Wang, {Qing Jun} and Andrej Sali and Rout, {Michael P.} and Chait, {Brian T.}",

note = "Publisher Copyright: {\textcopyright} 2015 Nature America, Inc.",

year = "2015",

month = dec,

day = "1",

doi = "10.1038/nmeth.3617",

language = "English",

volume = "12",

pages = "1135--1138",

number = "12",

}

TY - JOUR

T1 - A strategy for dissecting the architectures of native macromolecular assemblies

AU - Shi, Yi

AU - Pellarin, Riccardo

AU - Fridy, Peter C.

AU - Fernandez-Martinez, Javier

AU - Thompson, Mary K.

AU - Li, Yinyin

AU - Wang, Qing Jun

AU - Sali, Andrej

AU - Rout, Michael P.

AU - Chait, Brian T.

PY - 2015/12/1

Y1 - 2015/12/1

N2 - It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.

AB - It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.

UR - http://www.scopus.com/inward/record.url?scp=84949099771&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84949099771&partnerID=8YFLogxK

U2 - 10.1038/nmeth.3617

DO - 10.1038/nmeth.3617

M3 - Article

C2 - 26436480

AN - SCOPUS:84949099771

SN - 1548-7091

VL - 12

SP - 1135

EP - 1138

JO - Nature Methods

JF - Nature Methods

IS - 12

ER -

A strategy for dissecting the architectures of native macromolecular assemblies

Abstract

Bibliographical note

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this