Abstract
It remains particularly problematic to define the structures of native macromolecular assemblies, which are often of low abundance. Here we present a strategy for isolating complexes at endogenous levels from GFP-tagged transgenic cell lines. Using cross-linking mass spectrometry, we extracted distance restraints that allowed us to model the complexes' molecular architectures.
Original language | English |
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Pages (from-to) | 1135-1138 |
Number of pages | 4 |
Journal | Nature Methods |
Volume | 12 |
Issue number | 12 |
DOIs | |
State | Published - Dec 1 2015 |
Bibliographical note
Publisher Copyright:© 2015 Nature America, Inc.
ASJC Scopus subject areas
- Biotechnology
- Biochemistry
- Molecular Biology
- Cell Biology