A very stable cyclic DNA miniduplex with just two base pairs

Afaf H. El-Sagheer; Ravindra Kumar; Stuart Findlow; Joern M. Werner; Andrew N. Lane; Tom Brown

doi:10.1002/cbic.200700538

A very stable cyclic DNA miniduplex with just two base pairs

Afaf H. El-Sagheer, Ravindra Kumar, Stuart Findlow, Joern M. Werner, Andrew N. Lane, Tom Brown

Research output: Contribution to journal › Article › peer-review

55 Scopus citations

Abstract

(Chemical Equation Presented) What goes around... Cyclic mini-DNA duplexes, as shown in the scheme, have been synthesized in high yield on the multimicromole scale by using click chemistry. These B-like duplexes are very stable and bind to drugs, such as distamycin A and 7-aminoactinomycin D. Circular dichroism indicates that the bases are stacked, and UV melting and NMR spectroscopy showed that the interbase hydrogen bonds are very stable, even in a dimer.

Original language	English
Pages (from-to)	50-52
Number of pages	3
Journal	ChemBioChem
Volume	9
Issue number	1
DOIs	https://doi.org/10.1002/cbic.200700538
State	Published - Jan 4 2008

Keywords

Cyclic
DNA
DNA recognition
DNA structure
Miniduplex

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Organic Chemistry

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10.1002/cbic.200700538

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AU - Kumar, Ravindra

AU - Findlow, Stuart

AU - Werner, Joern M.

AU - Lane, Andrew N.

AU - Brown, Tom

PY - 2008/1/4

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N2 - (Chemical Equation Presented) What goes around... Cyclic mini-DNA duplexes, as shown in the scheme, have been synthesized in high yield on the multimicromole scale by using click chemistry. These B-like duplexes are very stable and bind to drugs, such as distamycin A and 7-aminoactinomycin D. Circular dichroism indicates that the bases are stacked, and UV melting and NMR spectroscopy showed that the interbase hydrogen bonds are very stable, even in a dimer.

AB - (Chemical Equation Presented) What goes around... Cyclic mini-DNA duplexes, as shown in the scheme, have been synthesized in high yield on the multimicromole scale by using click chemistry. These B-like duplexes are very stable and bind to drugs, such as distamycin A and 7-aminoactinomycin D. Circular dichroism indicates that the bases are stacked, and UV melting and NMR spectroscopy showed that the interbase hydrogen bonds are very stable, even in a dimer.

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KW - DNA

KW - DNA recognition

KW - DNA structure

KW - Miniduplex

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A very stable cyclic DNA miniduplex with just two base pairs

Abstract

Keywords

ASJC Scopus subject areas

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