A.actinomycetemcomitans-induced periodontal disease promotes systemic and local responses in rat periodontium

Beatriz De Brito Bezerra, Oelisoa Andriankaja, Jun Kang, Sandra Pacios, Hyung Jin Bae, Yu Li, Vincent Tsiagbe, Helen Schreiner, Daniel H. Fine, Dana T. Graves

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Aim To characterize the histologic and cellular response to A. actinomycetemcomitans (Aa) infection. Material & Methods Wistar rats infected with Aa were evaluated for antibody response, oral Aa colonization, loss of attachment, PMN recruitment, TNF-α in the junctional epithelium and connective tissue, osteoclasts and adaptive immune response in local lymph nodes at baseline and 4, 5 or 6 weeks after infection. Some groups were given antibacterial treatment at 4 weeks. Results An antibody response against Aa occurred within 4 weeks of infection, and 78% of inoculated rats had detectable Aa in the oral cavity (p < 0.05). Aa infection significantly increased loss of attachment that was reversed by antibacterial treatment (p < 0.05). TNF-α expression in the junctional epithelium followed the same pattern. Aa stimulated high osteoclast formation and TNF-α expression in the connective tissue (p < 0.05). PMN recruitment significantly increased after Aa infection (p < 0.05). Aa also increased the number of CD8 + T cells (p < 0.05), but not CD4 + T cells or regulatory T cells (Tregs) (p > 0.05). Conclusion Aa infection stimulated a local response that increased numbers of PMNs and TNF-α expression in the junctional epithelium and loss of attachment. Both TNF-α expression in JE and loss of attachment was reversed by antibiotic treatment. Aa infection also increased TNF-α in the connective tissue, osteoclast numbers and CD8 + T cells in lymph nodes. The results link Aa infection with important characteristics of periodontal destruction.

Original languageEnglish
Pages (from-to)333-341
Number of pages9
JournalJournal of Clinical Periodontology
Volume39
Issue number4
DOIs
StatePublished - Apr 2012

Keywords

  • A.actinomycetemcomitans
  • animals
  • bone resorption
  • disease models
  • host-pathogen interactions
  • periodontal disease

ASJC Scopus subject areas

  • Periodontics

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