ABCD2 identifies a subclass of peroxisomes in mouse adipose tissue

Xiaoxi Liu; Jingjing Liu; Joshua D. Lester; Sonja S. Pijut; Gregory A. Graf

doi:10.1016/j.bbrc.2014.11.046

ABCD2 identifies a subclass of peroxisomes in mouse adipose tissue

Xiaoxi Liu, Jingjing Liu, Joshua D. Lester, Sonja S. Pijut, Gregory A. Graf

Pharmaceutical Sciences

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

ATP-binding cassette transporter D2 (D2) is an ABC half transporter that is thought to promote the transport of very long-chain fatty acyl-CoAs into peroxisomes. Both D2 and peroxisomes increase during adipogenesis. Although peroxisomes are essential to both catabolic and anabolic lipid metabolism, their function, and that of D2, in adipose tissues remain largely unknown. Here, we investigated the D2 localization and the proteome of D2-containing organelles, in adipose tissue. Centrifugation of mouse adipose homogenates generated a fraction enriched with D2, but deficient in peroxisome markers including catalase, PEX19, and ABCD3 (D3). Electron microscopic imaging of this fraction confirmed the presence of D2 protein on an organelle with a dense matrix and a diameter of ∼200 nm, the typical structure and size of a microperoxisome. D2 and PEX19 antibodies recognized distinct structures in mouse adipose. Immunoisolation of the D2-containing compartment confirmed the scarcity of PEX19 and proteomic profiling revealed the presence of proteins associated with peroxisome, endoplasmic reticulum (ER), and mitochondria. D2 is localized to a distinct class of peroxisomes that lack many peroxisome proteins, and may associate physically with mitochondria and the ER.

Original language	English
Pages (from-to)	129-134
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	456
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2014.11.046
State	Published - Jan 2 2015

Bibliographical note

Funding Information:
This work was funded by grants from the National Institute of Diabetes and Digestive and Kidney Diseases (Grant DK080874 , DK100892 ). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We acknowledge the University of Kentucky Proteomics Core that is partially supported by grants from the National Cancer Institute ( P30CA177558 ) and the National Institute of General Medical Sciences ( P20GM103486 ) from the National Institutes of Health . The authors would like to acknowledge Jeannie Haak for assistance with manuscript preparation.

Publisher Copyright:
© 2014 Elsevier Inc. All rights reserved.

Keywords

ABC transporter
Adipose tissue
Peroxisome
Proteomics
Subcellular organelle

ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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10.1016/j.bbrc.2014.11.046

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title = "ABCD2 identifies a subclass of peroxisomes in mouse adipose tissue",

abstract = "ATP-binding cassette transporter D2 (D2) is an ABC half transporter that is thought to promote the transport of very long-chain fatty acyl-CoAs into peroxisomes. Both D2 and peroxisomes increase during adipogenesis. Although peroxisomes are essential to both catabolic and anabolic lipid metabolism, their function, and that of D2, in adipose tissues remain largely unknown. Here, we investigated the D2 localization and the proteome of D2-containing organelles, in adipose tissue. Centrifugation of mouse adipose homogenates generated a fraction enriched with D2, but deficient in peroxisome markers including catalase, PEX19, and ABCD3 (D3). Electron microscopic imaging of this fraction confirmed the presence of D2 protein on an organelle with a dense matrix and a diameter of ∼200 nm, the typical structure and size of a microperoxisome. D2 and PEX19 antibodies recognized distinct structures in mouse adipose. Immunoisolation of the D2-containing compartment confirmed the scarcity of PEX19 and proteomic profiling revealed the presence of proteins associated with peroxisome, endoplasmic reticulum (ER), and mitochondria. D2 is localized to a distinct class of peroxisomes that lack many peroxisome proteins, and may associate physically with mitochondria and the ER.",

keywords = "ABC transporter, Adipose tissue, Peroxisome, Proteomics, Subcellular organelle",

author = "Xiaoxi Liu and Jingjing Liu and Lester, {Joshua D.} and Pijut, {Sonja S.} and Graf, {Gregory A.}",

note = "Funding Information: This work was funded by grants from the National Institute of Diabetes and Digestive and Kidney Diseases (Grant DK080874 , DK100892 ). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We acknowledge the University of Kentucky Proteomics Core that is partially supported by grants from the National Cancer Institute ( P30CA177558 ) and the National Institute of General Medical Sciences ( P20GM103486 ) from the National Institutes of Health . The authors would like to acknowledge Jeannie Haak for assistance with manuscript preparation. Publisher Copyright: {\textcopyright} 2014 Elsevier Inc. All rights reserved.",

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AU - Pijut, Sonja S.

AU - Graf, Gregory A.

N1 - Funding Information: This work was funded by grants from the National Institute of Diabetes and Digestive and Kidney Diseases (Grant DK080874 , DK100892 ). The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. We acknowledge the University of Kentucky Proteomics Core that is partially supported by grants from the National Cancer Institute ( P30CA177558 ) and the National Institute of General Medical Sciences ( P20GM103486 ) from the National Institutes of Health . The authors would like to acknowledge Jeannie Haak for assistance with manuscript preparation. Publisher Copyright: © 2014 Elsevier Inc. All rights reserved.

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ABCD2 identifies a subclass of peroxisomes in mouse adipose tissue

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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