Accelerated regenerative neurite formation by a neuronal surface epitope reactive with the monoclonal antibody, Leu 7

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Abstract

A family of glycoproteins sharing an epitope with myelin associated glycoprotein as recognized by the monoclonal antibody Leu 7 (HNK-1) has been found to be present on neurons grown in culture from embryonic chicks and rats. Immunofluorescent staining demonstrates that, in vitro, 100% of the neurons from dorsal root ganglia and spinal cord from 7-8 day chick embryos react with Leu 7. Analysis of in vitro regenerative neurite formation by neurons on substrates enriched with Leu 7 showed accelerated regenerative process formation under limiting conditions. These results indicate that the Leu 7 epitope on neurons is appropriate for substrate adhesion and promotes rapid process extension.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalNeuroscience Letters
Volume56
Issue number2
DOIs
StatePublished - May 14 1985

Funding

This work was supported by the Multiple Sclerosis Society of Canada (J.R.) and the Medical Research Council of Canada (R.J.R.). R.M. is a fellow of the M.S. Society of Canada.

Funders
Multiple Sclerosis Society of Canada
Medical Research Council Canada

    Keywords

    • HNK-1
    • Leu 7
    • MAG
    • monoclonal antibody
    • myelin associated glycoprotein
    • neurite formation
    • neurons

    ASJC Scopus subject areas

    • General Neuroscience

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