Activation and peripheral expansion of murine T-cell receptor γδ intraepithelial lymphocytes

S. R. Guehler, J. A. Bluestone, T. A. Barrett

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Background and Aims: The intestinal epithelial compartment is populated by CD8+ αβ and γδ intraepithelial lymphocytes (IELs), which monitor the integrity of the epithelial barrier. αβ IELs are activated by peptide antigens presented by class I major histocompatibility complex (MHC) molecules, but it is unclear how γδ IELs are activated. Methods: G8 T-cell receptor (TCR) γδ transgenic (Tg) mice (specific for the class I MHC alloantigen, T22/10b) were crossed to class I MHC-deficient β2- microglobulin-knockout (β2m°) mice, and Tg+ IELs were examined for relative yields and surface and functional phenotype. Results: Evidence for class I MHC-induced activation of Tg+ IELs was supported by the detection of 4-fold greater numbers of Tg+ IELs in G8 x β2m+ mice that proliferated at 15-fold higher levels than IELs from G8 x β2m°mice. However, expression of CD69, production of cytokine (interleukin 2 and interferon gamma), and detection of cytolytic function for IELs in G8 x β2m°mice suggested that class I MHC was not required for γδ IEL development or maturation. Conclusions: These results suggest that CD8+ TCR γδ IELs do not require class I MHC for development but support the notion that antigens presented by class I MHC molecules are involved in the peripheral expansion and differentiation of this subset.

Original languageEnglish
Pages (from-to)327-334
Number of pages8
Issue number2
StatePublished - 1999

ASJC Scopus subject areas

  • Hepatology
  • Gastroenterology


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