Activation and stabilization of chymotrypsin in microdomains of poly(ethylenimine) derivatives. A model of in vivo environment

Junghun Suh; Young Sam Lee; Sanghwa Han

doi:10.1016/S0960-894X(98)00211-X

Activation and stabilization of chymotrypsin in microdomains of poly(ethylenimine) derivatives. A model of in vivo environment

Junghun Suh, Young Sam Lee, Sanghwa Han

Molecular and Cellular Biochemistry

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Both activity and stability of α-Chymotrypsin (ChT) are substantially enhanced in the microdomains of laurylated or benzylated derivatives of poly(ethylenimine). EPR data revealed that the enhancement in activity of ChT is due to increase in the polarity of the microenvironment of Ser-195 caused by complexation of ChT to the polymer derivatives.

Original language	English
Pages (from-to)	1331-1336
Number of pages	6
Journal	Bioorganic and Medicinal Chemistry Letters
Volume	8
Issue number	11
DOIs	https://doi.org/10.1016/S0960-894X(98)00211-X
State	Published - Jun 2 1998

Bibliographical note

Funding Information:
Acknowledgment. This work was supported by grants from Basic Research Institute Program (1997), Ministry of Education, and from KOSEF through Center for Molecular Catalysis at SNU.

ASJC Scopus subject areas

Biochemistry
Molecular Medicine
Molecular Biology
Pharmaceutical Science
Drug Discovery
Clinical Biochemistry
Organic Chemistry

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10.1016/S0960-894X(98)00211-X

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title = "Activation and stabilization of chymotrypsin in microdomains of poly(ethylenimine) derivatives. A model of in vivo environment",

abstract = "Both activity and stability of α-Chymotrypsin (ChT) are substantially enhanced in the microdomains of laurylated or benzylated derivatives of poly(ethylenimine). EPR data revealed that the enhancement in activity of ChT is due to increase in the polarity of the microenvironment of Ser-195 caused by complexation of ChT to the polymer derivatives.",

author = "Junghun Suh and Lee, {Young Sam} and Sanghwa Han",

note = "Funding Information: Acknowledgment. This work was supported by grants from Basic Research Institute Program (1997), Ministry of Education, and from KOSEF through Center for Molecular Catalysis at SNU.",

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AU - Suh, Junghun

AU - Lee, Young Sam

AU - Han, Sanghwa

N1 - Funding Information: Acknowledgment. This work was supported by grants from Basic Research Institute Program (1997), Ministry of Education, and from KOSEF through Center for Molecular Catalysis at SNU.

PY - 1998/6/2

Y1 - 1998/6/2

N2 - Both activity and stability of α-Chymotrypsin (ChT) are substantially enhanced in the microdomains of laurylated or benzylated derivatives of poly(ethylenimine). EPR data revealed that the enhancement in activity of ChT is due to increase in the polarity of the microenvironment of Ser-195 caused by complexation of ChT to the polymer derivatives.

AB - Both activity and stability of α-Chymotrypsin (ChT) are substantially enhanced in the microdomains of laurylated or benzylated derivatives of poly(ethylenimine). EPR data revealed that the enhancement in activity of ChT is due to increase in the polarity of the microenvironment of Ser-195 caused by complexation of ChT to the polymer derivatives.

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Activation and stabilization of chymotrypsin in microdomains of poly(ethylenimine) derivatives. A model of in vivo environment

Abstract

Bibliographical note

ASJC Scopus subject areas

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