Activation and up-regulation of translation initiation factor 4B contribute to arsenic-induced transformation

Yong Zhang, Qing Wang, Xiaoling Guo, Robert Miller, Yinglu Guo, Hsin Sheng Yang

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Arsenic is a known human carcinogen. However, the mechanism of how arsenic induces cell transformation remains unclear. In this study, we demonstrated that long-term exposure to sodium arsenite at low-dose (2μM) increases cell proliferation and neoplastic transformation in a mouse epidermal cell model, JB6 promotion-susceptible cells. The phosphorylation of AKT and its downstream targets, 70-kDa ribosomal protein S6 kinase (p70S6K) and translation initiation factor 4B (eIF4B), are increased in the arsenite treated cells, indicating that long-term arsenite treatment activates AKT-p70S6K signaling pathway. In addition, long-term exposure to arsenite up-regulates eIF4B expression and increases the rate of translation. Knockdown of eIF4B expression resulted in inhibition of arsenic-induced cell proliferation, transformation, and translation. Moreover, the expression of c-Myc which is up-regulated by long-term arsenite treatment is inhibited by eIF4B knockdown. Taken together, these results indicate that activation and up-regulation of eIF4B contributes to arsenic-induced transformation in JB6 cells.

Original languageEnglish
Pages (from-to)528-538
Number of pages11
JournalMolecular Carcinogenesis
Volume50
Issue number7
DOIs
StatePublished - Jul 2011

Keywords

  • Arsenic
  • C-Myc
  • EIF4B
  • Transformation

ASJC Scopus subject areas

  • Molecular Biology
  • Cancer Research

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