Abstract
The development of a procedure for the selection of a large number of functional antigen-binding B cells is described. Forty to 70% of the cells in the enriched population bind antigen, and the antigen-binding can be inhibited by pretreatment with either the free hapten (TNP-Lys) or anti-immunoglobulin. The enriched cells express both sIgM and sIgD and respond to mitogenic stimuli by proliferating and developing into antibody-forming cells. In contrast to normal spleen cells, however, the enriched cells also proliferate in response to sub-mitogenic levels of TNP-Brucella abortus and TNP-LPS.
Original language | English |
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Pages (from-to) | 607-613 |
Number of pages | 7 |
Journal | Journal of Immunology |
Volume | 130 |
Issue number | 2 |
State | Published - 1983 |
ASJC Scopus subject areas
- Immunology and Allergy
- Immunology