TY - JOUR
T1 - Activation of antigen-specific B cells
T2 - role of T cells, cytokines, and antigen in induction of growth and differentiation
AU - Noelle, R. J.
AU - Snow, E. C.
AU - Uhr, J. W.
AU - Vitetta, E. S.
PY - 1983
Y1 - 1983
N2 - T cells and cytokines were used to activate highly enriched populations of 2,4,6-trinitrophenyl (TNP)-binding B cells (TNP-ABC). TNP-ABC did not proliferate or differentiate when they were cultured with thymus-dependent (TD) antigen, even in the presence of supernatants known to contain B-cell growth and differentiation factors. However, purified TNP-ABC did proliferate and differentiate when they were cultured with TD antigen in the presence of carrier-primed T cells and antigen (TNP-keyhole limpet hemocyanin) i.e., linked recognition. TNP-ABC blasts generated under conditions of linked recognition proliferated and differentiated in response to cytokines in the absence of T cells and antigen. In contrast, under conditions of nonlinked recognition (hapten and carrier on different molecules) TNP-ABC blasts also proliferated but did not differentiate in response to the same cytokines. These results indicate that antigen-specific 'resting' B cells must be activated by T cells and antigen prior to becoming response to cytokines. Furthermore, activation under conditions of linked and nonlinked recognition generates two different types of blasts with regard to their subsequent response to cytokines.
AB - T cells and cytokines were used to activate highly enriched populations of 2,4,6-trinitrophenyl (TNP)-binding B cells (TNP-ABC). TNP-ABC did not proliferate or differentiate when they were cultured with thymus-dependent (TD) antigen, even in the presence of supernatants known to contain B-cell growth and differentiation factors. However, purified TNP-ABC did proliferate and differentiate when they were cultured with TD antigen in the presence of carrier-primed T cells and antigen (TNP-keyhole limpet hemocyanin) i.e., linked recognition. TNP-ABC blasts generated under conditions of linked recognition proliferated and differentiated in response to cytokines in the absence of T cells and antigen. In contrast, under conditions of nonlinked recognition (hapten and carrier on different molecules) TNP-ABC blasts also proliferated but did not differentiate in response to the same cytokines. These results indicate that antigen-specific 'resting' B cells must be activated by T cells and antigen prior to becoming response to cytokines. Furthermore, activation under conditions of linked and nonlinked recognition generates two different types of blasts with regard to their subsequent response to cytokines.
UR - http://www.scopus.com/inward/record.url?scp=0021027957&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0021027957&partnerID=8YFLogxK
U2 - 10.1073/pnas.80.21.6628
DO - 10.1073/pnas.80.21.6628
M3 - Article
C2 - 6605533
AN - SCOPUS:0021027957
VL - 80
SP - 6628
EP - 6631
IS - 21 I
ER -