Activation of hepatic NF-κB by phenobarbital in rats

Yixin Li, Lai K. Leung, Brett T. Spear, Howard P. Glauert

Research output: Contribution to journalArticlepeer-review

30 Scopus citations


Nuclear factor κB (NF-κB) is an important stress-induced transcription factor in many cell types. In this study we examined if the hepatic tumor promoting agent phenobarbital could activate NF-κB in the liver. Rats were fed 0.05% phenobarbital in the diet for 3, 6, or 10 days. Benzyloxyresorufin dealkylase activity was greatly increased in rats receiving phenobarbital. The DNA binding activity of NF-κB was determined using electrophoretic mobility shift assays. After 3 days, NF-κB activity was increased two-fold in rats receiving phenobarbital. NF-κB activity continued to increase at 6 and 10 days in rats fed phenobarbital, with a four-fold increase seen at day 10. Cold competition and antibody supershift experiments showed the DNA binding activity to be specific for NF-κB. These data show that phenobarbital activates hepatic NF-κB in rats.

Original languageEnglish
Pages (from-to)982-989
Number of pages8
JournalBiochemical and Biophysical Research Communications
Issue number3
StatePublished - Dec 24 1996

Bibliographical note

Funding Information:
These studies were supported by NIH Grants ES-05815 and CA-01688, by American Cancer Society Institutional Grant 163, and by the Kentucky Agricultural Experiment Station.

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology


Dive into the research topics of 'Activation of hepatic NF-κB by phenobarbital in rats'. Together they form a unique fingerprint.

Cite this