Activation of JNK and c-Jun is involved in glucose oxidase-mediated cell death of human lymphoma cells

Young Ok Son, Yong Suk Jang, Xianglin Shi, Jeong Chae Lee

Research output: Contribution to journalArticlepeer-review

12 Scopus citations


Mitogen-activated protein kinases (MAPK) affect the activation of activator protein-1 (AP-1), which plays an important role in regulating a range of cellular processes. However, the roles of these signaling factors on hydrogen peroxide (H2O2)-induced cell death are unclear. This study examined the effects of H2O2 on the activation of MAPK and AP-1 by exposing the cells to H2O2 generated by either glucose oxidase or a bolus addition. Exposing BJAB or Jurkat cells to H 2O2 affected the activities of MAPK differently according to the method of H2O2 exposure. H2O2 increased the AP-1-DNA binding activity in these cells, where continuously generated H2O2 led to an increase in mainly the c-Fos, FosB and c-Jun proteins. The c-Jun-NH2-terminal kinase (JNK)-mediated activation of c-Jun was shown to be related to the H2O 2-induced cell death. However, the suppression of H2O 2-induced oxidative stress by either JNK inhibitor or c-Jun specific antisense transfection was temporary in the cells exposed to glucose oxidase but not to a bolus H2O2. This was associated with the disruption of death signaling according to the severe and prolonged depletion of reduced glutathione. Overall, these results suggest that H2O 2 may decide differently the mode of cell death by affecting the intracellular redox state of thiol-containing antioxidants, and this depends more closely on the duration exposed to H2O2 than the concentration of this agent.

Original languageEnglish
Pages (from-to)545-551
Number of pages7
JournalMolecules and Cells
Issue number6
StatePublished - Dec 2009

Bibliographical note

Funding Information:
This work was supported by a Grant from the Korea Science and Engineering Foundation, Republic of Korea (No. 2009-0067712).


  • Activator protein-1
  • Antioxidant defense enzymes
  • Human lymphoma cells
  • Hydrogen peroxide
  • Mitogen-activated protein kinase

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology


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