Activation of syk in an immature B cell line does not require lyn activity

Subramanian Muthukkumar, Chandrasekar Venkataraman, Teriaka Woods, Subbarao Bondada

Research output: Contribution to journalArticlepeer-review

9 Scopus citations


BKS-2 is an immature B cell lymphoma that undergoes apoptotic cell death when signaled via its surface IgM receptor. To study the signaling components of surface IgM mediated apoptosis in B lymphoma cells, we generated mutants of BKS-2 that were resistant to anti-IgM induced apoptosis. One mutant cell line, 1.B5, did not undergo apoptotic cell death upon treatment with anti- IgM antibodies and also did not exhibit elevation of intracellular Ca2+ in response to cross-linking of surface IgM. This appeared to be due to a defect in protein tyrosine kinase (PTK) activity since fewer proteins were tyrosine phosphorylated in the mutant cells stimulated with anti-IgM when compared to wild type BKS-2. Subsequently, we showed that protein tyrosine kinases lyn and blk were inducibly tyrosine phosphorylated in the wild type BKS-2 but not in 1.B5 mutant cells in response to anti-IgM. Also the kinase activity of lyn was elevated in the wild type but not in mutant cells upon triggering through surface IgM. Furthermore, tyrosine phosphorylation of CD19, a known substrate of lyn, was inducible in anti-IgM stimulated BKS-2 cells but severely reduced in 1.B5 cells. In contrast, kinase activity of another src kinase, blk, was increased on anti-IgM stimulation in both wild type and mutant cells. Surprisingly, syk, a non-src protein tyrosine kinase important for surface IgM mediated signaling, was tyrosine phosphorylated in the lyn deficient mutant cells as well as in the wild type BKS-2 cells. Furthermore, anti-IgM induced increase in kinase activity of syk was similar in the mutant and wild type cells. Thus, in contrast to other studies that propose syk to be a downstream target of src family kinases, syk may act upstream of lyn in immature B cells. Consistent with a functional syk, its target, phospholipase γ2 (PLC-γ2) was normally tyrosine phosphorylated in mutant cells.

Original languageEnglish
Pages (from-to)865-875
Number of pages11
JournalMolecular Immunology
Issue number12-13
StatePublished - 1997

Bibliographical note

Funding Information:
Acknolliedgements-This work was supportedi n part by the grants AI 21490a nd AG 05731t o S. B. from the National Institutes of Health.


  • B cells
  • CD19
  • PLCγ2
  • Signaling
  • blk
  • lyn
  • sIgM
  • syk

ASJC Scopus subject areas

  • Immunology
  • Molecular Biology


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