As part of the on-line radiosynthesis of radiopharmaceuticals labeled with short-lived positron emitters, the dephosphorylation of an appropriate nucleotide to the corresponding nucleoside is required. The rapid dephosphorylation of thymidine monophosphate was accomplished using the enzyme alkaline phosphatase, which was covalently bound to a derivatized polysulfone membrane through a phthalate or succinyl linkage. A nearly quantitative conversion of thymidine was accomplished within a 2-3 minute period making this system amenable for use with carbon- 11 (hal flife=20.4 minutes) labeled nucleotides.
|Number of pages||11|
|Journal||Journal of Membrane Science|
|State||Published - Aug 1 1991|
Bibliographical noteFunding Information:
This work was supported in part by the National Science Foundation (Grant No. Rll-8110671) and the Commonwealth of Kentucky through the Kentucky EPSCoR program.
- alkaline phosphatase
- polysulfone membranes
ASJC Scopus subject areas
- Materials Science (all)
- Physical and Theoretical Chemistry
- Filtration and Separation