Activity of alkaline phosphatase bound to polysulfone membranes

Jeffrey A. Hughes; Shiqi Zhou; Dibakar Bhattacharyya; Michael Jay

doi:10.1016/S0376-7388(00)80325-7

Activity of alkaline phosphatase bound to polysulfone membranes

Jeffrey A. Hughes, Shiqi Zhou, Dibakar Bhattacharyya, Michael Jay

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

As part of the on-line radiosynthesis of radiopharmaceuticals labeled with short-lived positron emitters, the dephosphorylation of an appropriate nucleotide to the corresponding nucleoside is required. The rapid dephosphorylation of thymidine monophosphate was accomplished using the enzyme alkaline phosphatase, which was covalently bound to a derivatized polysulfone membrane through a phthalate or succinyl linkage. A nearly quantitative conversion of thymidine was accomplished within a 2-3 minute period making this system amenable for use with carbon- 11 (hal flife=20.4 minutes) labeled nucleotides.

Original language	English
Pages (from-to)	75-85
Number of pages	11
Journal	Journal of Membrane Science
Volume	60
Issue number	1
DOIs	https://doi.org/10.1016/S0376-7388(00)80325-7
State	Published - Aug 1 1991

Bibliographical note

Funding Information:
This work was supported in part by the National Science Foundation (Grant No. Rll-8110671) and the Commonwealth of Kentucky through the Kentucky EPSCoR program.

Funding

This work was supported in part by the National Science Foundation (Grant No. Rll-8110671) and the Commonwealth of Kentucky through the Kentucky EPSCoR program.

Funders	Funder number
National Science Foundation (NSF)

Keywords

alkaline phosphatase
polysulfone membranes
radiopharmaceuticals
thymidine

ASJC Scopus subject areas

Biochemistry
General Materials Science
Physical and Theoretical Chemistry
Filtration and Separation

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10.1016/S0376-7388(00)80325-7

Cite this

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title = "Activity of alkaline phosphatase bound to polysulfone membranes",

abstract = "As part of the on-line radiosynthesis of radiopharmaceuticals labeled with short-lived positron emitters, the dephosphorylation of an appropriate nucleotide to the corresponding nucleoside is required. The rapid dephosphorylation of thymidine monophosphate was accomplished using the enzyme alkaline phosphatase, which was covalently bound to a derivatized polysulfone membrane through a phthalate or succinyl linkage. A nearly quantitative conversion of thymidine was accomplished within a 2-3 minute period making this system amenable for use with carbon- 11 (hal flife=20.4 minutes) labeled nucleotides.",

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author = "Hughes, {Jeffrey A.} and Shiqi Zhou and Dibakar Bhattacharyya and Michael Jay",

note = "Funding Information: This work was supported in part by the National Science Foundation (Grant No. Rll-8110671) and the Commonwealth of Kentucky through the Kentucky EPSCoR program.",

year = "1991",

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doi = "10.1016/S0376-7388(00)80325-7",

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T1 - Activity of alkaline phosphatase bound to polysulfone membranes

AU - Hughes, Jeffrey A.

AU - Zhou, Shiqi

AU - Bhattacharyya, Dibakar

AU - Jay, Michael

N1 - Funding Information: This work was supported in part by the National Science Foundation (Grant No. Rll-8110671) and the Commonwealth of Kentucky through the Kentucky EPSCoR program.

PY - 1991/8/1

Y1 - 1991/8/1

N2 - As part of the on-line radiosynthesis of radiopharmaceuticals labeled with short-lived positron emitters, the dephosphorylation of an appropriate nucleotide to the corresponding nucleoside is required. The rapid dephosphorylation of thymidine monophosphate was accomplished using the enzyme alkaline phosphatase, which was covalently bound to a derivatized polysulfone membrane through a phthalate or succinyl linkage. A nearly quantitative conversion of thymidine was accomplished within a 2-3 minute period making this system amenable for use with carbon- 11 (hal flife=20.4 minutes) labeled nucleotides.

AB - As part of the on-line radiosynthesis of radiopharmaceuticals labeled with short-lived positron emitters, the dephosphorylation of an appropriate nucleotide to the corresponding nucleoside is required. The rapid dephosphorylation of thymidine monophosphate was accomplished using the enzyme alkaline phosphatase, which was covalently bound to a derivatized polysulfone membrane through a phthalate or succinyl linkage. A nearly quantitative conversion of thymidine was accomplished within a 2-3 minute period making this system amenable for use with carbon- 11 (hal flife=20.4 minutes) labeled nucleotides.

KW - alkaline phosphatase

KW - polysulfone membranes

KW - radiopharmaceuticals

KW - thymidine

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JF - Journal of Membrane Science

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Activity of alkaline phosphatase bound to polysulfone membranes

Abstract

Bibliographical note

Funding

Keywords

ASJC Scopus subject areas

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