TY - JOUR
T1 - Acute bronchial obstruction in sheep
T2 - Histopathology and gland cytokine expression
AU - Cox, Robert A.
AU - Burke, Ann S.
AU - Oliveras, Gloria
AU - Enkhbaatar, Perenlei
AU - Traber, Lillian D.
AU - Zwischenberger, Joseph B.
AU - Jeschke, Marc G.
AU - Schmalstieg, Frank C.
AU - Herndon, David N.
AU - Traber, Daniel L.
AU - Hawkins, Hal K.
PY - 2005/11
Y1 - 2005/11
N2 - An ovine model of smoke inhalation and burn (S+ B) injury models the pathophysiology of these injuries in humans. This study examines the degree of airway obstruction, associated histopathology, and bronchial gland cell expression of cytokines during the first 24 hours after S+ B injury in sheep. Changes in the mean degree of obstruction were limited to the bronchial airways, showing significant increases in obstruction with time, P< .05. At 4 hours after injury, the obstructive material was predominantly mucus, with neutrophils clustered around and within gland acini. At 8 to 24 hours, bronchial obstruction was characterized by increased inflammatory cell accumulation. Immunohistochemical results showed that gland cells constitutively express and secrete interleukin (IL)-1β, and that after injury there is an increase in the percentage of gland cells staining for IL-1α, IL-8, and tumor necrosis factor (TNF)-α, P < .05.
AB - An ovine model of smoke inhalation and burn (S+ B) injury models the pathophysiology of these injuries in humans. This study examines the degree of airway obstruction, associated histopathology, and bronchial gland cell expression of cytokines during the first 24 hours after S+ B injury in sheep. Changes in the mean degree of obstruction were limited to the bronchial airways, showing significant increases in obstruction with time, P< .05. At 4 hours after injury, the obstructive material was predominantly mucus, with neutrophils clustered around and within gland acini. At 8 to 24 hours, bronchial obstruction was characterized by increased inflammatory cell accumulation. Immunohistochemical results showed that gland cells constitutively express and secrete interleukin (IL)-1β, and that after injury there is an increase in the percentage of gland cells staining for IL-1α, IL-8, and tumor necrosis factor (TNF)-α, P < .05.
KW - Airway obstruction
KW - Cytokine
KW - Inflammation
KW - Mucous glands
KW - Mucus
KW - Neutrophils
UR - http://www.scopus.com/inward/record.url?scp=33646696188&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33646696188&partnerID=8YFLogxK
U2 - 10.1080/01902140600574967
DO - 10.1080/01902140600574967
M3 - Article
C2 - 16684715
AN - SCOPUS:33646696188
SN - 0190-2148
VL - 31
SP - 819
EP - 837
JO - Experimental Lung Research
JF - Experimental Lung Research
IS - 9-10
ER -